Huang Fan, Guo Genglin, Feng Lu, Cai Tongbo, Huang Xu
Department of Clinical Laboratory, Children's Hospital of Nanjing Medical University, Nanjing, China.
Shandong Institute of Sericulture, Jiaodong Innovation Center, Shandong Academy of Agricultural Sciences, Yantai, China.
J Glob Antimicrob Resist. 2025 Jan;40:90-95. doi: 10.1016/j.jgar.2024.11.009. Epub 2024 Dec 2.
Highly carbapenem-resistant Salmonella has emerged worldwide in recent years and is largely associated with the multiform transmission of resistance genes, which poses a huge challenge in clinical practice. Our study delves into the resistance mechanisms and epidemiology of bla-carrying plasmids.
Whole-genome sequencing was utilised to analyse the molecular characteristics and antimicrobial resistance mechanisms of Salmonella isolates recovered from the faeces of a paediatric patient at the Children's Hospital of Nanjing Medical University. Moreover, we conducted an epidemiologic analysis and focused on studying the mechanisms of plasmid-mediated bla transmission, incorporating genomes deposited in the NCBI Pathogen Detection database.
The clinical isolate 23S9 belonged to serovar Typhimurium, antigenic profile 4:i:-, ST34, and carried pNDM_23S9 harbouring several antimicrobial resistance genes, including aac(6')-Ib-cr6, OXA-1, catB3, arr-3, qacEdeltal and bla. Comparative analysis revealed that bla can exist in different plasmids of different isolates, proving its transmission through plasmids. Furthermore, bla-carrying isolates are mostly resistant to beta-lactams, aminoglycosides, sulphonamide, macrolides, and trimethoprim.
These findings provided thorough and intuitive insights into the intercontinental spread of bla-carrying Salmonella. Ongoing surveillance is essential for effectively monitoring the worldwide dissemination of this high-risk carbapenem-resistant Salmonella.
近年来，高碳青霉烯耐药性沙门氏菌在全球范围内出现，这在很大程度上与耐药基因的多种传播形式有关，给临床实践带来了巨大挑战。我们的研究深入探讨了携带bla质粒的耐药机制和流行病学。
利用全基因组测序分析从南京医科大学儿童医院一名儿科患者粪便中分离出的沙门氏菌菌株的分子特征和抗菌耐药机制。此外，我们进行了流行病学分析，并重点研究了质粒介导的bla传播机制，纳入了NCBI病原体检测数据库中保存的基因组。
临床分离株23S9属于鼠伤寒血清型，抗原谱4:i: -，ST34，携带含有多个抗菌耐药基因的pNDM_23S9，包括aac(6')-Ib-cr6、OXA-1、catB3、arr-3、qacEdeltal和bla。比较分析表明，bla可存在于不同分离株的不同质粒中，证明其通过质粒传播。此外，携带bla的分离株大多对β-内酰胺类、氨基糖苷类、磺胺类、大环内酯类和甲氧苄啶耐药。
这些发现为携带bla的沙门氏菌的洲际传播提供了全面而直观的见解。持续监测对于有效监测这种高风险碳青霉烯耐药性沙门氏菌在全球的传播至关重要。
