Zeng Wei, Luo Ming, Du Pengcheng, Li Zhenpeng, Peng Yao, Wang Mengyu, Zhao Wenxuan, Zhang Huayao, Li Yang, Luo Pengjie, Wu Yannong, Xu Jialiang, Li Xu, Lu Xin, Kan Biao
Yantai Center for Disease Control and Prevention, Yantai 264003, Shandong, China;National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.;School of Public Health, Shandong University, Jinan 250012, Shandong, China.
Yulin Center for Disease Control and Prevention, Yulin 537000, Guangxi, China.
Biomed Environ Sci. 2024 Nov 20;37(11):1252-1261. doi: 10.3967/bes2024.104.
Our study aimed to conduct genomic characterization of strains carrying the gene in the intestinal tract of healthy individuals. The objectives were to underscore the importance of genomic surveillance for drug resistance in both commensal and pathogenic bacteria among healthy populations, and to establish protocols for regulating drug resistance plasmids based on the completion of a comprehensive map of drug resistance plasmid genomes.
We performed antimicrobial susceptibility testing and employed second- and third-generation sequencing techniques to analyze strains harboring the gene, to surveil drug-resistant bacteria in the intestines of healthy subjects. Sequence comparison was conducted using both core- and pan-genome approaches. Concurrently, conjugation experiments were carried out to assess the efficiency of plasmid transfer.
We isolated a carbapenem-resistant enterica serovar Typhimurium strain from a healthy food worker in China. This strain harbored an IncHI2/IncHI2A plasmid carrying along with multiple antibiotic resistance genes (ARGs). Our findings highlight the potential for asymptomatic carriers to facilitate the transmission of ARGs. Pan-genomic analysis revealed that -positive plasmids could traverse bacterial species barriers, facilitating cross-host transmission.
This study marks the first detection of in strains isolated from healthy individuals. We underscore the risk associated with the transmission of conjugative hybrid plasmids carrying , which have the potential to be harbored and transmitted among healthy individuals. Enhanced surveillance of drug-resistant pathogens and plasmids in the intestinal microbiota of healthy individuals could provide insights into the risk of ARG transmission and pathways for population-wide dissemination ARG transfer factors.
我们的研究旨在对健康个体肠道中携带该基因的菌株进行基因组特征分析。目标是强调在健康人群中共生菌和病原菌耐药性基因组监测的重要性，并基于完成耐药性质粒基因组的全面图谱建立调控耐药性质粒的方案。
我们进行了抗菌药物敏感性测试，并采用第二代和第三代测序技术分析携带该基因的菌株，以监测健康受试者肠道中的耐药菌。使用核心基因组和泛基因组方法进行序列比较。同时，进行接合实验以评估质粒转移效率。
我们从中国一名健康食品工作者中分离出一株耐碳青霉烯类肠炎沙门氏菌鼠伤寒血清型菌株。该菌株携带一个IncHI2/IncHI2A质粒，该质粒携带该基因以及多个抗生素抗性基因（ARGs）。我们的研究结果突出了无症状携带者促进ARGs传播的可能性。泛基因组分析表明，该基因阳性质粒可跨越细菌种属屏障，促进跨宿主传播。
本研究首次在从健康个体分离的菌株中检测到该基因。我们强调了携带该基因的接合性杂交质粒传播相关的风险，这些质粒有可能在健康个体中携带和传播。加强对健康个体肠道微生物群中耐药病原体和质粒的监测，可为ARG传播风险及ARG转移因子在人群中传播途径提供见解。
