Heitkemper M M, Shaver J F
Am J Physiol. 1986 Apr;250(4 Pt 1):G546-52. doi: 10.1152/ajpgi.1986.250.4.G546.
The effects of three doses (5, 100, and 250 micrograms/kg) of pentagastrin on the activities of choline acetyltransferase (ChAT) and acetylcholine esterase (AChE), the neurotransmitter enzymes that synthesize and degrade acetylcholine, and monoamine oxidase (MAO), the degradation enzyme for catecholamines, were investigated. Enzyme activities were assayed in 6 gastrointestinal segments of 21- and 28-day-old and adult rats. All animals were injected intraperitoneally for 7 days with pentagastrin, and the results were compared with age-matched controls receiving saline for 7 days. Plasma and adrenal corticosterone levels were measured. No consistent differences in adrenocortical variables existed between pentagastrin- and saline-treated animals. Similarly, no consistent pentagastrin dose responses of ChAT, AChE, and MAO activities were evident. However, at the highest dose pentagastrin generally produced increases in ChAT activities in 21- and 28-day-old rats, while producing decreases in AChE and MAO activities in 21-day-old rats and increases in 28-day-old animals. There were few significant differences in enzyme activities in adult rats receiving pentagastrin as compared to saline.
研究了三种剂量（5、100和250微克/千克）的五肽胃泌素对胆碱乙酰转移酶（ChAT）和乙酰胆碱酯酶（AChE）活性的影响，这两种神经递质酶分别负责合成和降解乙酰胆碱，同时还研究了其对儿茶酚胺降解酶单胺氧化酶（MAO）活性的影响。在21日龄、28日龄和成年大鼠的6个胃肠道节段中测定酶活性。所有动物腹腔注射五肽胃泌素7天，并将结果与接受生理盐水注射7天的年龄匹配对照组进行比较。测量血浆和肾上腺皮质酮水平。五肽胃泌素处理组和生理盐水处理组动物的肾上腺皮质变量不存在一致差异。同样，ChAT、AChE和MAO活性也没有明显的五肽胃泌素剂量反应。然而，在最高剂量时，五肽胃泌素通常会使21日龄和28日龄大鼠的ChAT活性增加，同时使21日龄大鼠的AChE和MAO活性降低，使28日龄动物的AChE和MAO活性增加。与生理盐水处理组相比，接受五肽胃泌素的成年大鼠的酶活性几乎没有显著差异。
