Li Yalin, Hu Weiwei, Lin Bing, Ma Teng, Zhang Zhentian, Hu Weiqian, Zhou Rui, Kwok Lai-Yu, Sun Zhihong, Zhu Cuifeng, Zhang Heping
Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
Gut Microbes. 2024 Jan-Dec;16(1):2434675. doi: 10.1080/19490976.2024.2434675. Epub 2024 Dec 4.
Autism spectrum disorder (ASD) currently lacks effective diagnostic and therapeutic approaches. Disruptions in the gut ecosystem have been observed in individuals with ASD, suggesting that targeting gut microbiota through probiotic and dietary supplementation may serve as a potential treatment strategy. This two-phase study aimed to characterize the fecal metagenome of children with ASD and investigate the beneficial effects of a combined probiotic and medium-carbohydrate intervention in ASD. Fecal metagenomes of children with ASD were compared to those of typically developing children, revealing intestinal dysbiosis in ASD, characterized by reduced levels of sp. , and sp. along with increased predicted abundances of inosine, glutamate, xanthine, and methylxanthine. The gut bacteriome and phageome exhibited high cooperativity. In a 3-month pilot study, subsp. Probio-M8 (Probio-M8) was administered alongside a medium-carbohydrate diet to Chinese children with ASD. The primary endpoint was the Childhood Autism Rating Scale (CARS), while the secondary endpoint was the Gastrointestinal Symptom Rating Scale (GSRS). A total of 72 autistic children were initially recruited for the intervention study, but only 53 completed the intervention. Probio-M8, in combination with dietary intervention, significantly improved CARS and GSRS scores, increased fecal levels of , , , and sp. while also reducing (Benjamini-Hochberg corrected ≤ 0.05 for all cases). The intervention also modulated fecal metabolites associated with the metabolism of amino acids (lysine), neurotransmitters (glutamate, γ-aminobutyric acid), polyunsaturated fatty acids (arachidonate, myristic acid), and vitamin B. In conclusion, Probio-M8 combined with medium-carbohydrate diet effectively improved ASD symptoms, with associated changes in the gut microbiome and metabolome, supporting its potential as an adjunctive therapy for ASD.
自闭症谱系障碍（ASD）目前缺乏有效的诊断和治疗方法。在ASD患者中观察到肠道生态系统紊乱，这表明通过益生菌和饮食补充剂来靶向肠道微生物群可能是一种潜在的治疗策略。这项两阶段研究旨在表征ASD儿童的粪便宏基因组，并研究益生菌与中等碳水化合物联合干预对ASD的有益效果。将ASD儿童的粪便宏基因组与发育正常儿童的进行比较，发现ASD患者存在肠道生态失调，其特征是 菌属、 菌属水平降低，同时肌苷、谷氨酸、黄嘌呤和甲基黄嘌呤的预测丰度增加。肠道细菌群落和噬菌体群落表现出高度协同性。在一项为期3个月的试点研究中，将 亚种Probio-M8（Probio-M8）与中等碳水化合物饮食一起给予中国ASD儿童。主要终点是儿童自闭症评定量表（CARS），次要终点是胃肠道症状评定量表（GSRS）。共有72名自闭症儿童最初被招募参加干预研究，但只有53名完成了干预。Probio-M8与饮食干预相结合，显著改善了CARS和GSRS评分，增加了粪便中 菌属、 菌属、 菌属和 菌属的水平，同时也降低了 菌属（所有病例经本雅明尼-霍奇伯格校正 ≤ 0.05）。该干预还调节了与氨基酸（赖氨酸）、神经递质（谷氨酸、γ-氨基丁酸）、多不饱和脂肪酸（花生四烯酸、肉豆蔻酸）和维生素B代谢相关的粪便代谢物。总之，Probio-M8与中等碳水化合物饮食相结合有效地改善了ASD症状，同时肠道微生物组和代谢组也发生了相关变化，支持了其作为ASD辅助治疗的潜力。
