Gavino V C, Vinet B, David F, Garneau M, Brunengraber H
Anal Biochem. 1986 Feb 1;152(2):256-61. doi: 10.1016/0003-2697(86)90407-0.
The concentration of acetone dissolved in liver perfusion medium was determined by injection of the sample into a gas chromatograph equipped with a Carbopack/Carbowax-packed glass column. Interference from labile acetoacetate which readily decomposes to acetone was eliminated by treating the samples with NaBH4 prior to the analysis. Acetone was detected and quantified as 2-propanol. Separation of labeled 2-propanol in the sample by high-performance liquid chromatography allowed the determination of its specific activity. These methods make possible the convenient and rapid determination of acetone concentration and specific activity in biological samples.
将样品注入配备Carbopack/Carbowax填充玻璃柱的气相色谱仪中，测定溶解在肝脏灌注培养基中的丙酮浓度。在分析前，通过用NaBH4处理样品，消除了易分解为丙酮的不稳定乙酰乙酸的干扰。丙酮被检测并定量为2-丙醇。通过高效液相色谱法分离样品中的标记2-丙醇，可以测定其比活性。这些方法使得方便、快速地测定生物样品中的丙酮浓度和比活性成为可能。
