通过气相色谱顶空分析法同时测定生物样品中的酮体。
Simultaneous determination of ketone bodies in biological samples by gas chromatographic headspace analysis.
作者信息
López-Soriano F J, Argilés J M
出版信息
J Chromatogr Sci. 1985 Mar;23(3):120-3. doi: 10.1093/chromsci/23.3.120.
A method is described on gas chromatographic headspace analysis for the determination of the physiological ketone bodies: acetone, acetoacetate and beta-hydroxybutyrate. The procedure proposed involves quantitative conversion of both acetoacetate by decarboxylation in a strong acidic medium, and beta-hydroxybutyrate by oxidation to acetoacetate and decarboxylation to acetone, which is then quantified chromatographically. The successful application of the method is illustrated by the values of ketone bodies in plasma, kidney, lung, and liver of fed and 48-hr-fasted rats.
描述了一种用于测定生理性酮体（丙酮、乙酰乙酸和β-羟基丁酸）的气相色谱顶空分析法。所提出的程序包括在强酸性介质中通过脱羧对乙酰乙酸进行定量转化，以及通过氧化成乙酰乙酸并脱羧成丙酮对β-羟基丁酸进行定量转化，然后对丙酮进行色谱定量。通过喂食和禁食48小时的大鼠血浆、肾脏、肺和肝脏中酮体的值说明了该方法的成功应用。
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