Zhang Jiajia, Du Gaigai, Zhang Guoyun, Zhang Jianguo, Diao Songfeng
Key Laboraltory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Changsha, China.
Research Institute of Non-timber Forestry, Chinese Academy of Forestry, Key Laboratory of Non-timber Forest Germplasm Enhancement and Utilization of National Forestry and Grassland Administration, Zhengzhou, China.
Front Plant Sci. 2024 Nov 20;15:1487960. doi: 10.3389/fpls.2024.1487960. eCollection 2024.
The basic helix-loop-helix (bHLH) transcription factor family is one of the largest gene families in plants, extensively involved in plant growth, organ development, and stress responses. However, limited studies of this family are available in sea buckthorn ().
In this study, we identified 144 bHLH genes in (HrbHLH) through a genome-wide search method, then explored their DNA and protein sequences and physicochemical properties.
According to the sequence similarities, we classified them into 15 groups with specific motif structures. To explore their expressions, we performed gene expression profiling using RNA-Seq and identified 122 HrbHLH mRNAs were highly expressed, while the remaining 22 HrbHLH genes were expressed at very low levels in all 21 samples. Among these HrbHLH genes, showed the highest expression level in the root nodule, root, leaf, stem and fruit tissues. Furthermore, eleven HrbHLH genes displayed increased expressions during the fruit development process of sea buckthorn. Finally, we validated the expression patterns of HrbHLH genes using reverse transcription quantitative real-time PCR (QPCR). This comprehensive analysis provides a useful esource that enables further investigation of the physiological roles and molecular functions of the HrbHLH TFs.
基本螺旋-环-螺旋（bHLH）转录因子家族是植物中最大的基因家族之一，广泛参与植物生长、器官发育和应激反应。然而，沙棘中对该家族的研究有限。
在本研究中，我们通过全基因组搜索方法在沙棘（HrbHLH）中鉴定出144个bHLH基因，然后探索它们的DNA和蛋白质序列以及理化性质。
根据序列相似性，我们将它们分为15个具有特定基序结构的组。为了探究它们的表达情况，我们使用RNA-Seq进行基因表达谱分析，鉴定出122个HrbHLH mRNA高表达，而其余22个HrbHLH基因在所有21个样本中表达水平极低。在这些HrbHLH基因中， 在根瘤、根、叶、茎和果实组织中表达水平最高。此外，11个HrbHLH基因在沙棘果实发育过程中表达增加。最后，我们使用逆转录定量实时PCR（QPCR）验证了HrbHLH基因的表达模式。这种全面分析提供了一个有用的资源，能够进一步研究HrbHLH转录因子的生理作用和分子功能。
