Godang Noel L, Nguyen Anita D, DeMeis Jeffrey D, Paudel Sunita S, Campbell Nick J, Barnes Kingston J, Jeon Kahyeon, Roussell Alayla S, Gregson Kimberly A, Borchert Glen M
Pharmacology, University of South Alabama College of Medicine, Mobile, AL.
Computer Science, University of South Alabama School of Computing, Mobile, AL.
MicroPubl Biol. 2024 Nov 19;2024. doi: 10.17912/micropub.biology.001332. eCollection 2024.
The excision of specific tRNA-derived small RNAs (tsRNAs), yRNA-derived small RNAs (ysRNAs) and ribosomal RNA-derived small RNAs (rsRNAs) is now well established. Several reports have suggested many of these fragments function much like traditional microRNAs (miRNAs). That said, whereas the expressions of the majority of appreciably expressed miRNAs in HCT116 colon cancer cells are significantly decreased in individual knockouts (KOs) of DROSHA, DGCR8, XPO5, and DICER, on average, only 3.5% of tsRNA, ysRNA, and rsRNA expressions are impaired. Conversely, tsRNA, ysRNA, and rsRNA expressions are significantly increased in each of these KOs as compared to WT. As such, although DICER has been suggested to be involved with the expression of specific tsRNAs, ysRNAs, and rsRNAs, our study finds no evidence supporting the involvement of any of these canonical miRNA biogenesis enzymes in their expressions.
目前，特定的tRNA衍生小RNA（tsRNA）、yRNA衍生小RNA（ysRNA）和核糖体RNA衍生小RNA（rsRNA）的切除已得到充分证实。多项报告表明，这些片段中的许多功能与传统的微小RNA（miRNA）非常相似。也就是说，虽然在HCT116结肠癌细胞中，大多数明显表达的miRNA的表达在DROSHA、DGCR8、XPO5和DICER的单个基因敲除（KO）中显著降低，但平均而言，只有3.5%的tsRNA、ysRNA和rsRNA表达受到影响。相反，与野生型相比，这些基因敲除中的每一个中tsRNA、ysRNA和rsRNA的表达均显著增加。因此，尽管有人认为DICER参与特定tsRNA、ysRNA和rsRNA的表达，但我们的研究没有发现证据支持这些经典miRNA生物合成酶中的任何一种参与它们的表达。
