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一种具有大斯托克斯位移的靶向溶酶体的荧光探针，用于可视化生物硫醇和。 （原文最后“and.”表述不完整，可能影响准确理解）

A lysosome-targeted fluorescent probe with large Stokes shift for visualizing biothiols and .

作者信息

Zhao Taotao, Zhang Tong, Tao Zijun, Zhou Zhe, Xia Xiaofeng, Wu Zhengjun, Wang Feiyi, Ren Jun, Wang Erfei

机构信息

Hubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei University, Wuhan 430062, P.R. China.

出版信息

iScience. 2024 Nov 7;27(12):111334. doi: 10.1016/j.isci.2024.111334. eCollection 2024 Dec 20.

DOI:10.1016/j.isci.2024.111334
PMID:39634562
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11615578/
Abstract

Lysosomal biothiols play critical roles in numerous cellular processes and diseases. Researching an effective method for real-time labeling biothiols in lysosomes is of great significance and urgency, as it could provide essential information for the diagnosis of relevant diseases. In this study, we developed a lysosome-targeted fluorescent probe (LY-DCM-P) with a large Stokes shift of 150 nm for the sensitive and selective detection of biothiols and . Additionally, LY-DCM-P showed low cytotoxicity and excellent lysosome-targeted ability. The probe was successfully employed to monitor fluctuations in lysosomal biothiols in various living systems, enabling enormous potential to accurately monitor the occurrence and progress of biothiol-related diseases.

摘要

溶酶体生物硫醇在众多细胞过程和疾病中发挥着关键作用。研究一种在溶酶体中实时标记生物硫醇的有效方法具有重大意义和紧迫性，因为它可为相关疾病的诊断提供重要信息。在本研究中，我们开发了一种具有150 nm大斯托克斯位移的溶酶体靶向荧光探针（LY-DCM-P），用于灵敏且选择性地检测生物硫醇。此外，LY-DCM-P表现出低细胞毒性和出色的溶酶体靶向能力。该探针已成功用于监测各种生物体系中溶酶体生物硫醇的波动情况，具有准确监测生物硫醇相关疾病发生和进展的巨大潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff8f/11615578/98369ee9a069/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff8f/11615578/0f62eecb6689/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff8f/11615578/8f8941f47364/sc1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff8f/11615578/0ab9f0124945/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff8f/11615578/5f6ed28f9945/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff8f/11615578/7fd4bef166bf/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff8f/11615578/62ac2d9f2293/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff8f/11615578/2cebbf29581f/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff8f/11615578/98369ee9a069/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff8f/11615578/0f62eecb6689/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff8f/11615578/8f8941f47364/sc1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff8f/11615578/0ab9f0124945/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff8f/11615578/5f6ed28f9945/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff8f/11615578/7fd4bef166bf/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff8f/11615578/62ac2d9f2293/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff8f/11615578/2cebbf29581f/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff8f/11615578/98369ee9a069/gr6.jpg

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