利用基于人群的房室模型和视黄醇同位素稀释法研究加纳育龄妇女的维生素A动力学和全身储备情况。

Use of Population-Based Compartmental Modeling and Retinol Isotope Dilution to Study Vitamin A Kinetics and Total Body Stores among Ghanaian Women of Reproductive Age.

作者信息

Green Michael H, Lopez-Teros Veronica, Green Joanne Balmer, Lietz Georg, Kumordzie Sika M, Oxley Anthony, Fuseini Ahmed D, Nyaaba K Winifred, Becher Emily, Davis Jennie N, Wessells K Ryan, Adu-Afarwuah Seth, Engle-Stone Reina, Haskell Marjorie J

机构信息

Department of Nutritional Sciences, The Pennsylvania State University, University Park, PA, United States.

Department of Chemical and Biological Sciences, Universidad de Sonora, Hermosillo, Sonora, Mexico.

出版信息

Curr Dev Nutr. 2024 Oct 18;8(11):104484. doi: 10.1016/j.cdnut.2024.104484. eCollection 2024 Nov.

BACKGROUND

Limited data are available on vitamin A kinetics and total body stores (TBS) in women. Such information can be obtained using compartmental modeling and retinol isotope dilution (RID).

OBJECTIVES

Objectives were to apply population-based ("super-subject") modeling to determine retinol kinetics in nonpregnant Ghanaian women of reproductive age and to use RID to predict TBS in the group and its individuals.

METHODS

Women ( 89) ingested a dose of [H]retinyl acetate and blood samples (3/woman) were collected from 6 h to 91 d, with all participants sampled at 14 d, about half at either 21 or 28 d, and each at one other time. Composite data (plasma retinol fraction of dose; FD) were analyzed using Simulation, Analysis and Modeling software to obtain kinetic parameters, TBS, and other state variables as well as model-derived values for the RID composite coefficient . The latter were used in the RID equation TBS (μmol) = × 1/SA (where SA is plasma retinol specific activity) to predict TBS at various times.

RESULTS

Model-predicted TBS was 973 μmol ( 87). Geometric mean RID-predicted TBS was 965, 926, and 1006 μmol at 14, 21, and 28 d, respectively, with wide ranges [for example, 252-3848 μmol on day 14 ( 86)]; TBS predictions were similar at later times. Participants had a mean 2 y of vitamin A in stores and estimated liver vitamin A concentrations in the normal range. Model-predicted vitamin A disposal rate was 1.3 μmol/d and plasma recycling number was 37.

CONCLUSIONS

Super-subject modeling provides an estimate of group mean TBS as well as group-specific values for the RID coefficient ; the latter can be used to confidently predict TBS by RID for individual participants in the group under study or in similar individuals at 14 d or more after isotope ingestion.

TRIAL REGISTRATION NUMBER

Trial is registered (NCT04632771) at https://clinicaltrials.gov.

背景

关于女性维生素A动力学和全身储存量（TBS）的数据有限。此类信息可通过房室模型和视黄醇同位素稀释法（RID）获得。

目的

目的是应用基于人群的（“超级受试者”）模型来确定加纳育龄非孕女性的视黄醇动力学，并使用RID预测该组及其个体的TBS。

方法

89名女性摄入一剂[H]醋酸视黄酯，并在6小时至91天内采集血样（每人3份），所有参与者在第14天采样，约一半在第21天或第28天采样，每人在另一个时间点采样。使用模拟、分析和建模软件分析综合数据（剂量的血浆视黄醇分数；FD），以获得动力学参数、TBS和其他状态变量以及RID综合系数的模型推导值。后者用于RID方程TBS（微摩尔）=×1/SA（其中SA是血浆视黄醇比活性），以预测不同时间的TBS。

结果

模型预测的TBS为973微摩尔（标准差87）。几何平均RID预测的TBS在第14、21和28天分别为965、926和1006微摩尔，范围较宽[例如，第14天为252 - 3848微摩尔（标准差86）]；后期的TBS预测相似。参与者储存的维生素A平均可供2年使用，估计肝脏维生素A浓度在正常范围内。模型预测的维生素A处置率为1.3微摩尔/天，血浆循环次数为37次。