Grosman Arieh, Duanis-Assaf Tal, Mazurski Noa, Zektzer Roy, Frydendahl Christian, Stern Liron, Reches Meital, Levy Uriel
Department of Applied Physics, The Benin School of Engineering and Computer Science, The Hebrew University of Jerusalem, 91904, Jerusalem, Israel.
The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, 91904, Jerusalem, Israel.
Nanophotonics. 2023 Mar 31;12(14):2831-2839. doi: 10.1515/nanoph-2022-0722. eCollection 2023 Jul.
Coronavirus disease 2019 (COVID-19) is a newly emerging human infectious disease that continues to develop new variants. A crucial step in the quest to reduce the infection is the development of rapid and reliable virus detectors. Here, we report a chip scale photonic sensing device consisting of a silicon-nitride double microring resonator (MRR) for detecting SARS-CoV-2 in clinical samples. The sensor is implemented by surface activation of one of the MRRs, acting as a probe, with DNA primers for SARS-CoV-2 RNA, whereas the other MRR is used as a reference. The performance of the sensor is determined by applying different amounts of SARS-CoV-2 complementary RNA. As will be shown in the paper, our device detects the RNA fragments at concentrations of 10 cp/μL and with sensitivity of 750 nm/RIU. As such, it shows a promise toward the implementation of label-free, small form factor, CMOS compatible biosensor for SARS-CoV-2, which is also environment, temperature, and pressure independent. Our approach can also be used for detecting other SARS-CoV-2 genes, as well as other viruses and pathogens.
2019冠状病毒病（COVID-19）是一种新出现的人类传染病，不断出现新的变种。寻求减少感染的关键一步是开发快速可靠的病毒检测设备。在此，我们报告一种芯片级光子传感装置，它由一个用于检测临床样本中严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的氮化硅双微环谐振器（MRR）组成。该传感器通过对作为探针的其中一个MRR进行表面激活来实现，该MRR带有针对SARS-CoV-2 RNA的DNA引物，而另一个MRR用作参考。通过施加不同量的SARS-CoV-2互补RNA来确定传感器的性能。正如本文将展示的，我们的装置能够检测浓度为10拷贝/微升的RNA片段，灵敏度为750纳米/折射率单位。因此，它有望实现用于SARS-CoV-2的无标记、小尺寸、与互补金属氧化物半导体（CMOS）兼容的生物传感器，该传感器也不受环境、温度和压力的影响。我们的方法还可用于检测其他SARS-CoV-2基因以及其他病毒和病原体。
