Electrical & Computer Engineering, Rice University, Houston, Texas 77005, United States.
Department of Bioengineering, Rice University, Houston, Texas 77005, United States.
ACS Sens. 2022 Aug 26;7(8):2253-2261. doi: 10.1021/acssensors.2c00756. Epub 2022 Aug 8.
Real-time detection of biomarkers, particularly nitric oxide (NO), is of utmost importance for critical healthcare monitoring, therapeutic dosing, and fundamental understanding of NO's role in regulating many physiological processes. However, detection of NO in a biological medium is challenging due to its short lifetime and low concentration. Here, we demonstrate for the first time that photonic microring resonators (MRRs) can provide real-time, direct, and detection of NO in a mouse wound model. The MRR encodes the NO concentration information into its transfer function in the form of a resonance wavelength shift. We show that these functionalized MRRs, fabricated using complementary metal oxide semiconductor (CMOS) compatible processes, can achieve sensitive detection of NO (sub-μM) with excellent specificity and no apparent performance degradation for more than 24 h of operation in biological medium. With alternative functionalizations, this compact lab-on-chip optical sensing platform could support real-time detection of myriad of biochemical species.
实时检测生物标志物，特别是一氧化氮（NO），对于关键的医疗保健监测、治疗剂量和对 NO 调节许多生理过程的作用的基本理解至关重要。然而，由于其半衰期短和浓度低，在生物介质中检测 NO 具有挑战性。在这里，我们首次证明光子微环谐振器（MRR）可以实时、直接地检测小鼠伤口模型中的 NO。MRR 将 NO 浓度信息以共振波长移动的形式编码到其传递函数中。我们表明，这些使用互补金属氧化物半导体（CMOS）兼容工艺制造的功能化 MRR 可以实现对 NO 的灵敏检测（亚微摩尔），具有优异的特异性，并且在生物介质中运行超过 24 小时后性能没有明显下降。通过替代功能化，这个紧凑的片上实验室光学传感平台可以支持实时检测无数的生化物质。
