Wang Jingyang, Wang Xin, Xiu Weiye, Li Chenchen, Yu Shiyou, Zhu Haobin, Yang Chenxi, Zhou Kechi, Ma Yongqiang
Key Laboratory of Cereal Food and Cereal Resources in Heilongjiang Province, School of Food Engineering, Harbin University of Commerce, Harbin 150028, China.
Keshan Branch of Heilongjiang Academy of Agricultural Sciences, Keshan 161601, Heilongjiang, China.
Food Funct. 2025 Jan 2;16(1):133-146. doi: 10.1039/d4fo04195j.
Chronic fatigue syndrome (CFS) is a long-term chronic condition that predisposes individuals to oxidative stress and disruption of the gut microbiota. In this study, sweet corn cob polysaccharide selenium nanoparticles (U-SCPSeNPs) with relatively small particle sizes were prepared using an ultrasound-assisted method. Transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDX), X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR), and ultraviolet-visible spectroscopy (UV-Vis) were used to characterize the U-SCPSeNPs and determine the monosaccharide composition of the U-SCPSeNPs. The U-SCPSeNPs were used to improve the CFS of the mice. The results showed that the ultrasound-assisted method reduced the particle size of the SeNPs, and U-SCPSeNPs with a particle size of 76.74 nm and a selenium content of 186.83 ± 7.80 mg g were obtained at an ultrasonication time of 40 min. Sweet corn cob (SCP) bound to the SeNPs through hydrogen bonding. In terms of energy production, the production capacity of Na-K-ATP, Mg-ATP, and Ca-ATP was enhanced by U-SCPSeSCP in CFS mice; In terms of oxidative stress, the levels of SOD and MDA were decreased and CAT and GSH-Px were increased by SCPSeSCP. U-SCPSeSCP improved the diversity and abundance of the gut microbiota in CFS mice, and decreasing the relative abundance of increased the relative abundance of at the phylum level. This study provides a reference for synthesizing polysaccharide SeNPs and assessing the ability of U-SCPSeNPs to alleviate CFS.
慢性疲劳综合征（CFS）是一种长期慢性疾病，使个体易患氧化应激和肠道微生物群紊乱。在本研究中，采用超声辅助法制备了粒径相对较小的甜玉米芯多糖硒纳米颗粒（U-SCPSeNPs）。利用透射电子显微镜（TEM）、能量色散X射线光谱（EDX）、X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）和紫外可见光谱（UV-Vis）对U-SCPSeNPs进行表征，并确定其单糖组成。将U-SCPSeNPs用于改善小鼠的慢性疲劳综合征。结果表明，超声辅助法减小了硒纳米颗粒的粒径，在超声处理40 min时获得了粒径为76.74 nm、硒含量为186.83±7.80 mg/g的U-SCPSeNPs。甜玉米芯（SCP）通过氢键与硒纳米颗粒结合。在能量产生方面，U-SCPSeSCP提高了CFS小鼠中Na-K-ATP、Mg-ATP和Ca-ATP的产生能力；在氧化应激方面，SCPSeSCP降低了超氧化物歧化酶（SOD）和丙二醛（MDA）水平，提高了过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）水平。U-SCPSeSCP改善了CFS小鼠肠道微生物群的多样性和丰度，在门水平上降低了[某菌]的相对丰度，增加了[另一菌]的相对丰度。本研究为合成多糖硒纳米颗粒及评估U-SCPSeNPs缓解慢性疲劳综合征的能力提供了参考。
