Gaunt S D, Pierce K R
Am J Vet Res. 1986 Apr;47(4):906-9.
Hematopoietic and marrow adherent cells were examined in 5 ovariohysterectomized dogs treated once with 1.5 mg of 17 beta-estradiol cyclopentylpropionate/kg of body weight. All dogs initially developed thrombocytopenia and neutrophilic leukocytosis, with megakaryocytopenia and an increased myeloid/erythroid ratio of the marrow. Later, 2 dogs became thrombocytopenic and neutropenic, with megakaryocytopenia, decreased myeloid/erythroid ratio, and increased concentration of plasma cells and mast cells in the marrow. The concentration of marrow granulocyte-macrophage colony-forming units (CFU-GM) was decreased at 2 and 3 weeks in all dogs after estradiol treatment. The concentration of marrow fibroblast CFU-F was decreased in all estradiol-treated dogs at 1 and 2 weeks. The ability of marrow adherent cells to support CFU-GM colony formation was unchanged in estradiol-treated dogs. Estradiol sulfate at final concentrations of 10(-8) to 10(-4)M did not affect the in vitro growth of CFU-GM and CFU-F, or the hematopoietic ability of marrow adherent cells from nontreated dogs.
对5只卵巢子宫切除的犬进行了研究，这些犬每千克体重单次注射1.5毫克环戊丙酸17β-雌二醇。所有犬最初均出现血小板减少和中性粒细胞增多，伴有巨核细胞减少和骨髓髓系/红系比例增加。之后，2只犬出现血小板减少和中性粒细胞减少，伴有巨核细胞减少、髓系/红系比例降低以及骨髓浆细胞和肥大细胞浓度增加。雌二醇治疗后2周和3周，所有犬的骨髓粒细胞-巨噬细胞集落形成单位（CFU-GM）浓度均降低。在1周和2周时，所有接受雌二醇治疗的犬的骨髓成纤维细胞CFU-F浓度均降低。在接受雌二醇治疗的犬中，骨髓贴壁细胞支持CFU-GM集落形成的能力未发生改变。终浓度为10(-8)至10(-4)M的硫酸雌二醇不影响CFU-GM和CFU-F的体外生长，也不影响未治疗犬骨髓贴壁细胞的造血能力。
