Wolosiuk R A, Hertig C M, Busconi L
Arch Biochem Biophys. 1986 Apr;246(1):1-8. doi: 10.1016/0003-9861(86)90443-1.
Kinetic analysis of glyceraldehyde-3-phosphate dehydrogenase showed that the enhancement of the NADP-linked activity by specific chloroplast modulators is a concerted process; either a selected second metabolite or the couple dithiothreitol/thioredoxin-f lowers the concentration of primary modulators (ATP, NADPH, inorganic phosphate, 1,3-diphosphoglycerate) required for maximal stimulation (A0.5). Organic solvents also stimulate NADP-glyceraldehyde-3-phosphate dehydrogenase in the absence of any modulator; the concentration for the highest specific activity correlates inversely with the respective octanol-water partition coefficient. On the other hand, alcohols also enhance enzyme activity by lowering the A0.5 for primary modulators. Another compound--spermine--inhibits both the ATP- and the inorganic phosphate-mediated activation, but it does not influence the NADPH-induced process.
对3-磷酸甘油醛脱氢酶的动力学分析表明，特定叶绿体调节剂对NADP相关活性的增强是一个协同过程；所选的第二种代谢物或二硫苏糖醇/硫氧还蛋白-f的组合会降低最大刺激（A0.5）所需的主要调节剂（ATP、NADPH、无机磷酸盐、1,3-二磷酸甘油酸）的浓度。在没有任何调节剂的情况下，有机溶剂也会刺激NADP-3-磷酸甘油醛脱氢酶；最高比活性的浓度与各自的正辛醇-水分配系数呈负相关。另一方面，醇类也通过降低主要调节剂的A0.5来增强酶活性。另一种化合物——精胺——会抑制ATP和无机磷酸盐介导的激活，但不影响NADPH诱导的过程。
