Kurzynska Aleksandra, Mierzejewski Karol, Golubska Monika, Jastrzebski Jan Pawel, Bogacka Iwona
Department of Animal Anatomy and Physiology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
Department of Plant Physiology, Genetics and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
BMC Genomics. 2024 Dec 5;25(1):1183. doi: 10.1186/s12864-024-11083-7.
The myometrium is involved in many processes during pregnancy and the estrous/menstrual cycle. Peroxisome proliferator-activated receptors (PPARs) can be regulators of the processes occurring in the myometrium. In the present study, we determined the global transcriptome profile of the porcine myometrium during the peri-implantation period and the late luteal phase of the estrous cycle. In addition, we investigated for the first time the influence of PPARγ ligands on the transcriptome profile.
The myometrium of gilts (n = 3) was collected on days 10-11 and 14-15 of pregnancy and on the corresponding days of the estrous cycle. The expression of PPARγ was confirmed in the tissue. Based on the mRNA level, further studies were conducted on myometrial explants obtained from pigs at days 14-15 of pregnancy and the corresponding days of the estrous cycle. The tissue sections were incubated in vitro for 6 h in the presence of a PPARγ agonist, pioglitazone (P; 10 µM), or antagonist, T0070907 (T; 1 µM). To identify the transcription profile of the myometrium, RNA-Seq was performed on the NovaSeq 6000 Illumina platform. This study identified 1082 differentially expressed genes (DEGs; 609 upregulated and 473 downregulated) in the porcine myometrium on days 14-15 of pregnancy compared with the corresponding days of the estrous cycle. During pregnancy, we detected 6 and 80 DEGs related to PPARγ agonist and antagonist, respectively. During the estrous cycle, we identified 4 and 17 DEGs for P and T vs. the control, respectively.
The results indicate that the DEGs are involved in a number of processes, including the immune response, prostaglandin synthesis, cell differentiation and communication. In addition, the role of PPARγ activity in regulating the expression of genes related to the immune response and hormone synthesis in the porcine myometrium has been demonstrated.
子宫肌层参与妊娠及发情/月经周期中的许多过程。过氧化物酶体增殖物激活受体（PPARs）可能是子宫肌层中发生的这些过程的调节因子。在本研究中，我们确定了猪子宫肌层在植入前期和发情周期黄体后期的整体转录组概况。此外，我们首次研究了PPARγ配体对转录组概况的影响。
在妊娠第10 - 11天和14 - 15天以及发情周期的相应天数收集后备母猪（n = 3）的子宫肌层。在组织中证实了PPARγ的表达。基于mRNA水平，对妊娠第14 - 15天的猪以及发情周期相应天数的子宫肌层外植体进行了进一步研究。将组织切片在PPARγ激动剂吡格列酮（P；10 μM）或拮抗剂T0070907（T；1 μM）存在的情况下体外孵育6小时。为了鉴定子宫肌层的转录谱，在Illumina NovaSeq 6000平台上进行了RNA测序。本研究确定，与发情周期的相应天数相比，妊娠第14 - 15天的猪子宫肌层中有1082个差异表达基因（DEGs；609个上调和473个下调）。在妊娠期间，我们分别检测到6个和80个与PPARγ激动剂和拮抗剂相关的DEGs。在发情周期中，我们分别鉴定出P和T与对照相比的4个和17个DEGs。
结果表明，这些DEGs参与了许多过程，包括免疫反应、前列腺素合成、细胞分化和通讯。此外，已证明PPARγ活性在调节猪子宫肌层中与免疫反应和激素合成相关基因的表达方面的作用。
