Zhou Yaoyao, Wang Yaqi, Li Fangli, Shi Yiming, Wu Taotao, Li Yingshuai
College of Basic Medical Sciences, Zhejiang Chinese Medical University, Zhejiang, 310053, China.
National Institute of Traditional Chinese Medicine Constitution and Preventive Treatment of Diseases, Beijing University of Chinese Medicine, Beijing, 100029, China.
Lipids Health Dis. 2024 Dec 5;23(1):399. doi: 10.1186/s12944-024-02379-4.
Klotho, an anti-aging protein, is linked to energy metabolism. There is limited research on the association of serum klotho and triglyceride glucose (TyG) index-related indicators. Our research aims to investigate the relationship of serum klotho with TyG-BMI (body mass index), TyG-WC (waist circumference), and TyG-WHtR (waist-to-height ratio).
From 2007 to 2016, we examined 6,370 participants in the National Health and Nutrition Examination Survey (NHANES). The enzyme-linked immunosorbent assay (ELISA) was utilized to measure serum klotho. We calculated the TyG-BMI, TyG-WC, and TyG-WHtR based on fasting triglycerides, fasting glucose, BMI, WC, and WHtR. Multiple linear regression analysis was used to evaluate the association of serum klotho with TyG-BMI, TyG-WC, and TyG-WHtR. Additionally, generalized additive model (GAM) and smoothing curves were used to evaluate the linear and nonlinear relationships. A piecewise regression model was also utilized to test for threshold effects and determine the breakpoints. Finally, the potential independent associations of serum klotho with TyG-BMI, TyG-WC, and TyG-WHtR were further explored using subgroup analysis.
We observed a statistically significant difference in serum klotho levels across different quartiles of the population. Based on the multiple linear regression analysis, serum klotho levels were negatively associated with TyG-related indicators. There was a nonlinear relationship between the serum klotho and TyG-BMI, TyG-WC, and TyG-WHtR. The segmented regression analysis revealed that the breakpoints of TyG-BMI, TyG-WC, and TyG-WHtR were 5.42, 6.67, and 1.89, respectively. Subgroup analysis showed that TyG-related indicators interacted with gender and diabetes.
In this study, a negative and nonlinear relationship was identified between serum klotho and TyG-related indicators. Further research is needed to clarify the potential mechanisms that may link serum klotho to TyG-BMI, TyG-WC, and TyG-WHtR.
衰老抑制蛋白α-klotho与能量代谢相关。关于血清α-klotho与甘油三酯葡萄糖（TyG）指数相关指标之间关联的研究有限。我们的研究旨在探讨血清α-klotho与TyG-BMI（体重指数）、TyG-WC（腰围）和TyG-WHtR（腰高比）之间的关系。
2007年至2016年期间，我们对国家健康与营养检查调查（NHANES）中的6370名参与者进行了检查。采用酶联免疫吸附测定（ELISA）法测量血清α-klotho。我们根据空腹甘油三酯、空腹血糖、BMI、WC和WHtR计算TyG-BMI、TyG-WC和TyG-WHtR。采用多元线性回归分析评估血清α-klotho与TyG-BMI、TyG-WC和TyG-WHtR之间的关联。此外，使用广义相加模型（GAM）和平滑曲线评估线性和非线性关系。还采用分段回归模型检验阈值效应并确定断点。最后，通过亚组分析进一步探讨血清α-klotho与TyG-BMI、TyG-WC和TyG-WHtR之间潜在的独立关联。
我们观察到不同四分位数人群的血清α-klotho水平存在统计学显著差异。基于多元线性回归分析，血清α-klotho水平与TyG相关指标呈负相关。血清α-klotho与TyG-BMI、TyG-WC和TyG-WHtR之间存在非线性关系。分段回归分析显示，TyG-BMI、TyG-WC和TyG-WHtR的断点分别为5.42、6.67和1.89。亚组分析表明，TyG相关指标与性别和糖尿病存在相互作用。
在本研究中，我们发现血清α-klotho与TyG相关指标之间存在负向非线性关系。需要进一步研究以阐明可能将血清α-klotho与TyG-BMI、TyG-WC和TyG-WHtR联系起来的潜在机制。
