Sun Rong, Chao Chen, Yu Jinglin, Copeland Les, Wang Shujun
State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, China.
School of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin 300457, China.
J Agric Food Chem. 2025 Jan 22;73(3):2103-2113. doi: 10.1021/acs.jafc.4c07046. Epub 2024 Dec 5.
Resistant starch (RS) has been shown to modulate intestinal microbiota in animal models in ways that could reduce the effects of dysbiosis-related diseases. However, the mechanism of how this is achieved is not understood. The present study aimed to reveal the mechanism of how RS mitigates dextran sulfate sodium (DSS)-induced colitis in mice by using a starch-lipid complex (RS type 5), with an RS type 2 from high-amylose maize starch as a comparison. Both RS5 and RS2 induced changes in the diversity and composition of the gut bacteria, leading to the alleviation of the induced colitis symptoms including decreasing the loss in body weight, disease activity index score, and colonic shortening. The levels of inflammatory cytokines were modulated and accompanied by an increase in goblet cell numbers and thickening of the intestinal mucus layer. RS5 was more effective, compared to RS2, in alleviating all of the colitis symptoms, mainly through improving the gut microflora dysbiosis and stimulating the generation of short-chain fatty acids (SCFAs). Our study shows that RS5 could effectively alleviate the symptoms of colitis, highlighting a potential use for RS5, particularly in relieving inflammatory bowel disease.
抗性淀粉（RS）已被证明在动物模型中可调节肠道微生物群，其方式可能减轻与生态失调相关疾病的影响。然而，实现这一过程的机制尚不清楚。本研究旨在通过使用淀粉-脂质复合物（5型RS），以高直链玉米淀粉中的2型RS作为对照，揭示RS减轻小鼠葡聚糖硫酸钠（DSS）诱导的结肠炎的机制。RS5和RS2均诱导肠道细菌多样性和组成的变化，从而减轻诱导的结肠炎症状，包括减轻体重减轻、疾病活动指数评分和结肠缩短。炎症细胞因子水平得到调节，并伴随着杯状细胞数量增加和肠道黏液层增厚。与RS2相比，RS5在减轻所有结肠炎症状方面更有效，主要是通过改善肠道微生物群失调和刺激短链脂肪酸（SCFA）的产生。我们的研究表明，RS5可有效减轻结肠炎症状，突出了RS5的潜在用途，特别是在缓解炎症性肠病方面。
