Li Yan, Han Chao, Shi Lili, Chen Chen, Zhao Jinpeng, Liu Tingting, Zhuo Qin
Key Laboratory of Trace Element Nutrition of National Health Commission (NHC), National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing, China.
J Appl Toxicol. 2025 Apr;45(4):646-658. doi: 10.1002/jat.4733. Epub 2024 Dec 5.
The transgenic maize DBN9936 × DBN9501, which confers resistance to insects and tolerance to herbicides, was developed via conventional cross breeding of transgenic maize DBN9936 and DBN9501. In our present study, a 90-day feeding toxicity study was conducted on Sprague Dawley rats to evaluate the safety of the maize. A total of 140 rats were randomly assigned to seven groups (n = 10/sex/group): one control group, three genetically modified (GM) groups with 17.5%, 35%, and 70% (wt/wt) GM maize, respectively, and three non-GM groups with corresponding incorporation rate of parental maize DBN318. The rats of control group were fed with AIN93G diet. The parameters including body weights, food consumption, hematology, serum biochemistry, organ weights, and histopathology were examined during the course of the study. Compared with the non-GM group or AIN93G control group, minor statistical differences were observed for some parameters in some groups, yet none of them was considered a GM-related adverse effect. In conclusion, the results demonstrated that no adverse effect was observed on rats following 90 days feeding with diet containing up to 70% GM maize. The results indicated that stacked maize DBN9936 × DBN9501 was as safe as its parental DBN318 maize.
转基因玉米DBN9936×DBN9501具有抗虫和耐除草剂特性，它是通过转基因玉米DBN9936和DBN9501的常规杂交培育而成。在我们目前的研究中，对斯普拉格-道利大鼠进行了为期90天的喂养毒性研究，以评估该玉米的安全性。总共140只大鼠被随机分为七组（每组雌雄各10只）：一组对照组、三组转基因（GM）组，分别喂食含17.5%、35%和70%（重量/重量）转基因玉米的饲料，以及三组非转基因组，分别喂食亲本玉米DBN318且配比与前面对应。对照组大鼠喂食AIN93G饲料。在研究过程中，检测了包括体重、食物摄入量、血液学指标、血清生化指标、器官重量和组织病理学等参数。与非转基因组或AIN93G对照组相比，部分组的一些参数存在微小的统计学差异，但均未被认为是与转基因相关的不良反应。总之，结果表明，用含高达70%转基因玉米的饲料喂养大鼠90天后，未观察到不良反应。结果表明，复合转基因玉米DBN9936×DBN9501与其亲本DBN318玉米一样安全。
