Li Zhiqian, Pu Mei, Zhou Peng, Zhang Tao, Xu Yang, Zhang Yusui
Department of Radiology, First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, China.
Turk J Gastroenterol. 2024 Nov 28;35(12):900-908. doi: 10.5152/tjg.2024.23558.
BACKGROUND/AIMS: The prevalence of rectal cancer is increasing every year due to changes in living and eating habits. Early diagnosis contributes to the treatment and survival of patients. This study investigated the feasibility of employing SLC26A4-AS1 combined with magnetic resonance imaging (MRI) for diagnosing rectal cancer.
The current study involved 125 patients with rectal cancer and an equal number of healthy individuals. The study focused on assessing the relationship between SLC26A4-AS1 expression and clinical data among patients with rectal cancer by analyzing the expression levels. MRI blood perfusion parameters (Ktrans, Kep, Ve, and incremental area under the curve (iAUC)) were measured in the patients with rectal cancer. The regulation of SLC26A4-AS1 on the biological function of rectal cancer cells was analyzed by Cell Counting Kit-8 (CCK-8) method, flow cytometry, and Transwell assay. Furthermore, luciferase activity assays and RNA-binding protein immunoprecipitation assay (RIP) were conducted to elucidate the relationship between SLC26A4-AS1 and microRNA-3174 (miR-3174).
A significant reduction in SLC26A4-AS1 expression was observed in rectal cancer alongside a significant increase in miR-3174 levels. SLC26A4-AS1 expression was negatively correlated with Ktrans and Kep values, but not with Ve or iAUC values. Cell experiments confirmed the inhibitory effect of SLC26A4-AS1 overexpression on the growth of rectal cancer cells. Additionally, SLC26A4-AS1 sponged miR-3174 mediated the progression of rectal cancer. The enriched miR-3174 may counteract the suppression of the biological activity of oe-SLC26A4-AS1 on rectal cancer cells.
SLC26A4-AS1 may serve as a diagnostic tool for rectal cancer, mediating tumor progression by directly targeting miR-3174.
背景/目的：由于生活和饮食习惯的改变，直肠癌的患病率逐年上升。早期诊断有助于患者的治疗和生存。本研究探讨了采用SLC26A4-AS1联合磁共振成像（MRI）诊断直肠癌的可行性。
本研究纳入125例直肠癌患者和同等数量的健康个体。通过分析表达水平，重点评估直肠癌患者中SLC26A4-AS1表达与临床数据之间的关系。测量直肠癌患者的MRI血流灌注参数（Ktrans、Kep、Ve和曲线下增量面积（iAUC））。采用细胞计数试剂盒-8（CCK-8）法、流式细胞术和Transwell实验分析SLC26A4-AS1对直肠癌细胞生物学功能的调控作用。此外，进行荧光素酶活性测定和RNA结合蛋白免疫沉淀测定（RIP），以阐明SLC26A4-AS1与微小RNA-3174（miR-3174）之间的关系。
直肠癌患者中SLC26A4-AS1表达显著降低，同时miR-3174水平显著升高。SLC26A4-AS1表达与Ktrans和Kep值呈负相关，但与Ve或iAUC值无关。细胞实验证实SLC26A4-AS1过表达对直肠癌细胞生长具有抑制作用。此外，SLC26A4-AS1吸附miR-3174介导了直肠癌的进展。富集的miR-3174可能抵消过表达SLC26A4-AS1对直肠癌细胞生物学活性的抑制作用。
SLC26A4-AS1可能作为直肠癌的诊断工具，通过直接靶向miR-3174介导肿瘤进展。
