Nakamura A, Osada H, Sakaguchi T, Sakurai I, Yagura S
Aviat Space Environ Med. 1986 Mar;57(3):256-62.
Regulatory mechanisms of erythrocyte glycolysis and 2,3-diphosphoglycerate (2,3DPG) metabolism under hypoxia were studied in rats exposed to a simulated altitude of 18,000 ft (5,486 m) for 5 d. Changes in erythrocyte glycolytic intermediates were determined by enzymatic analysis. Marked alterations of glycolytic intermediates were found during 1 d of exposure which were quite different from those observed during exposure for 2, 3, and 5 d. Alterations of intermediates seem to be highly correlated with blood pH changes; however, pH alone cannot explain the overall changes in intermediates. Results suggested that overall intermediate changes are the results of the combined effect of alterations of cellular pH and hemoglobin desaturation. Increased 2,3DPG at initial stages of exposure (within 1 d) may be caused mainly by the increased cellular pH; sustained elevation of 2,3DPG at later stages could be attributed to the relief of product inhibition of diphosphoglycerate mutase by deoxygenation.
对暴露于模拟海拔18,000英尺（5,486米）环境5天的大鼠，研究了低氧状态下红细胞糖酵解及2,3-二磷酸甘油酸（2,3-DPG）代谢的调节机制。通过酶分析测定红细胞糖酵解中间产物的变化。在暴露1天时发现糖酵解中间产物有显著改变，这与暴露2天、3天和5天时观察到的变化有很大不同。中间产物的改变似乎与血液pH变化高度相关；然而，仅pH值并不能解释中间产物的总体变化。结果表明，中间产物的总体变化是细胞pH改变和血红蛋白去饱和共同作用的结果。暴露初期（1天内）2,3-DPG增加可能主要是由于细胞pH升高；后期2,3-DPG持续升高可归因于脱氧作用缓解了二磷酸甘油酸变位酶的产物抑制。
