Jang Joo Hyun, Chang Yeok Boo, Kim Sang Min, Han Kisoo, Sim Wan-Sup, Hong Ki-Bae, Suh Hyung Joo, Han Sung Hee
Department of Integrated Biomedical and Life Science, Graduate School, Korea University, Seoul 02841, Republic of Korea.
Transdisciplinary Major in Learning Health Systems, Department of Healthcare Sciences, Graduate School, Korea University, Seoul 02841, Republic of Korea.
Food Funct. 2025 Jan 20;16(2):720-730. doi: 10.1039/d4fo04237a.
This study investigated the effects of on alleviating loperamide-induced constipation. To evaluate the efficacy of in Sprague-Dawley (SD) rats, fecal parameters, the intestinal transit rate, and changes in intestinal mucosal cells were measured through histological analysis. Additionally, serotonin levels, water absorption, tight junction-related gene expression, and the cecal short-chain fatty acid (SCFA) content were analyzed. The administration of significantly altered the fecal weight and moisture content and improved gastrointestinal transit in rats with loperamide-induced constipation. Furthermore, supplementation restored the thickness of both muscular and mucosal layers that had been reduced by loperamide and significantly increased the area of intestinal cells, including Cajal and crypt cells. administration upregulated the expression levels of tryptophan hydroxylase and aquaporin genes, which were downregulated by loperamide. As the dose of increased, there was a corresponding upregulation in the expression of tight junction-related genes, including occludin (), zonula occludens 1 (), and claudin 1 (). Additionally, the levels of c-kit, AQP 3, and OCLN proteins, which were elevated by loperamide treatment, were reduced with higher concentrations of . Loperamide decreased the acetic acid content; however, high doses of increased it, leading to a significant increase in the total cecal SCFA content. Thus, shows potential as a probiotic for improving constipation.
本研究调查了[具体物质]对缓解洛哌丁胺引起的便秘的作用。为评估[具体物质]对斯普拉格-道利（SD）大鼠的疗效，通过组织学分析测量了粪便参数、肠道转运速率以及肠道黏膜细胞的变化。此外，还分析了血清素水平、水分吸收、紧密连接相关基因表达以及盲肠短链脂肪酸（SCFA）含量。给予[具体物质]显著改变了洛哌丁胺诱导便秘大鼠的粪便重量和水分含量，并改善了胃肠道转运。此外，补充[具体物质]恢复了因洛哌丁胺而变薄的肌层和黏膜层厚度，并显著增加了包括 Cajal 细胞和隐窝细胞在内的肠道细胞面积。给予[具体物质]上调了被洛哌丁胺下调的色氨酸羟化酶和水通道蛋白基因的表达水平。随着[具体物质]剂量增加，紧密连接相关基因的表达相应上调，包括闭合蛋白（）、闭合小环蛋白 1（）和紧密连接蛋白 1（）。此外，经洛哌丁胺处理后升高的 c-kit、AQP 3 和 OCLN 蛋白水平，在较高浓度的[具体物质]作用下降低。洛哌丁胺降低了乙酸含量；然而，高剂量的[具体物质]使其增加，导致盲肠总 SCFA 含量显著增加。因此，[具体物质]显示出作为改善便秘的益生菌的潜力。
