Yamada Y, Serrero G
Biochem Biophys Res Commun. 1986 Mar 13;135(2):533-40. doi: 10.1016/0006-291x(86)90027-6.
The teratoma-derived insulin-independent cell line 1246-3A produces and secretes polypeptide mitogens in its culture serum-free medium. Mitogenic activities were separated by Sephadex G-50 chromatography into two fractions eluted, one in the void volume region, another one with an apparent Mr of 6 kDa. Only the 6 kDa mitogen presents properties similar to pancreatic insulin as estimated by radioimmunoassay, radio-receptor assay, and biochemical characterization. As a consequence, this factor is called insulin-related factor (IRF). Evidence presented in this paper indicate that ectopic IRF binds to insulin receptors on the producer cells, 1246-3A and acts in an autostimulatory manner.
源自畸胎瘤的非胰岛素依赖细胞系1246-3A在其无血清培养基中产生并分泌多肽促有丝分裂原。通过葡聚糖凝胶G-50柱层析将促有丝分裂活性分离为两个洗脱组分，一个在空体积区域洗脱，另一个表观分子量为6 kDa。通过放射免疫测定、放射受体测定和生化特性分析估计，只有6 kDa的促有丝分裂原具有与胰腺胰岛素相似的特性。因此，该因子被称为胰岛素相关因子（IRF）。本文提供的证据表明，异位IRF与产生细胞1246-3A上的胰岛素受体结合，并以自刺激方式发挥作用。
