Verbeek W, Bokemeyer C, Falk H, Schmoll H J
Medizinische Hochschule Hannover, Abteilung Hämatologie-Onkologie, Federal Republic of Germany.
J Cancer Res Clin Oncol. 1988;114(6):553-8. doi: 10.1007/BF00398176.
The in vitro growth requirements of three human embryonal carcinoma cell lines (H 12.7, 2102 EP, 1428 A) were investigated. The basal medium DME/F12 supplemented with insulin, transferrin, and low-density and high-density lipoproteins was sufficient to support substantial multiplication of all three lines. The most efficient attachment factor was either fibronectin (for 2102 EP and 1428 A) or collagen type I (H 12.7). In a serum-free system the influence of epidermal growth factor (EGF), insulin-like growth factor I, multiplication stimulating activity (MSA), a platelet extract, and the glucocorticoids dexamethasone and hydrocortisone, as determined by the DNA synthesis rate of the cells, was generally minimal. However, the DNA synthesis rate of cell lines H 12.7 and 2102 EP was increased by MSA, and the line with the highest potential to differentiate (H 12.7) was stimulated by EGF. All three cell lines secreted growth factors in a heterologous stimulation assay. Insulin-like growth factors I and II were not part of the growth promoting activity. The inhibitory effect of a monoclonal anti-EGF antibody on the 3H-thymidine incorporation of cell line 2102 EP might indicate autocrine secretion of EGF or an EGF-like factor by this cell line.
对三种人胚胎癌细胞系（H 12.7、2102 EP、1428 A）的体外生长需求进行了研究。添加胰岛素、转铁蛋白以及低密度和高密度脂蛋白的基础培养基DME/F12足以支持所有这三种细胞系大量增殖。最有效的黏附因子要么是纤连蛋白（对2102 EP和1428 A而言），要么是I型胶原（H 12.7）。在无血清体系中，根据细胞的DNA合成速率确定，表皮生长因子（EGF）、胰岛素样生长因子I、增殖刺激活性因子（MSA）、血小板提取物以及糖皮质激素地塞米松和氢化可的松的影响通常很小。然而，MSA提高了细胞系H 12.7和2102 EP的DNA合成速率，而具有最高分化潜能的细胞系（H 12.7）受到EGF的刺激。在异源刺激试验中，所有三种细胞系均分泌生长因子。胰岛素样生长因子I和II并非促生长活性的组成部分。单克隆抗EGF抗体对细胞系2102 EP的3H-胸腺嘧啶核苷掺入的抑制作用可能表明该细胞系自分泌EGF或一种EGF样因子。
