Das B R, Kanungo M S
Biochem Int. 1986 Feb;12(2):303-11.
Conformational changes in the chromatin of the cerebral hemisphere of 3-, 14- and 30-day old developing rats were studied before and after its ADP-ribosylation using DNase I and micrococcal nuclease (MNase). The rate and extent of digestion of chromatin by DNase I are the highest at 3-day and decline progressively thereafter. The rate and extent of digestion by MNase do not change during development. ADP-ribosylation of chromosomal proteins was carried out by incubating nuclei with NAD+ for 30 min and was followed by endonuclease digestion. Both the rate and extent of digestion by DNase I and MNase were enhanced after ADP-ribosylation which was the maximum for 3-day rats.
在3日龄、14日龄和30日龄发育中大鼠大脑半球染色质进行ADP核糖基化前后，使用脱氧核糖核酸酶I（DNase I）和微球菌核酸酶（MNase）研究了其构象变化。DNase I对染色质的消化速率和程度在3日龄时最高，此后逐渐下降。MNase的消化速率和程度在发育过程中没有变化。通过将细胞核与烟酰胺腺嘌呤二核苷酸（NAD+）孵育30分钟进行染色体蛋白的ADP核糖基化，然后进行核酸内切酶消化。ADP核糖基化后，DNase I和MNase的消化速率和程度均增强，其中3日龄大鼠增强幅度最大。
