Das B R, Kanungo M S
Mol Biol Rep. 1986;11(2):63-8. doi: 10.1007/BF00364815.
In vitro ADP-ribosylation of chromosomal proteins and its modulation by spermine, 3-aminobenzamide (3-AB) and benzamide were studied by incubating the nuclei of cerebral hemisphere of 3-, 14- and 30-day old rats with 32P-NAD+. Histones get ADP-ribosylated more than the non-histone chromosomal (NHC) proteins. H1 is the major target for ADP-ribosylation. Among the nucleosomal histones, H2B is ADP-ribosylated most. The other core histones also get ADP-ribosylated to a lesser extent. ADP-ribosylation of both histones and NHC proteins decreases during development. Spermine stimulates, whereas 3-AB and benzamide inhibit, 32P-ADP-ribose incorporation into histones and NHC proteins. These effects decrease with development. Mild digestion of chromatin by micrococcal nuclease (MNase), EcoRI and AluI prior to ADP-ribosylation stimulates incorporation of 32P-ADP-ribose. The degree of stimulation decreases as development proceeds. Such alterations indicate progressive condensation of chromatin with development.
通过用32P-NAD +孵育3日龄、14日龄和30日龄大鼠大脑半球的细胞核，研究了染色体蛋白的体外ADP-核糖基化及其受精胺、3-氨基苯甲酰胺（3-AB）和苯甲酰胺的调节。组蛋白比非组蛋白染色体（NHC）蛋白更容易发生ADP-核糖基化。H1是ADP-核糖基化的主要靶点。在核小体组蛋白中，H2B的ADP-核糖基化程度最高。其他核心组蛋白的ADP-核糖基化程度也较低。组蛋白和NHC蛋白的ADP-核糖基化在发育过程中减少。精胺刺激，而3-AB和苯甲酰胺抑制32P-ADP-核糖掺入组蛋白和NHC蛋白。这些作用随着发育而减弱。在ADP-核糖基化之前，用微球菌核酸酶（MNase）、EcoRI和AluI对染色质进行轻度消化可刺激32P-ADP-核糖的掺入。随着发育的进行，刺激程度降低。这种变化表明染色质随着发育而逐渐浓缩。
