Chen Taoran, Gao Yinze, Chen Xingtao, Dong Yajing, Wang Shiqian, Huang Qiao, Lin Shaorong, Wang Jiaxin, Liao Ming, Fan Huiying
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, China; National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, China.
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, China; National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, China.
Int J Biol Macromol. 2025 Jan;287:138405. doi: 10.1016/j.ijbiomac.2024.138405. Epub 2024 Dec 4.
The continuous circulation and evolution of the H5N6 subtype highly pathogenic avian influenza virus (HPAIV) challenge the development of the global poultry industry and human public health security. To address the potential threat of the H5N6 virus, a secure and efficacious vaccine is urgent. In our research, a self-assembling nanoparticle vaccine presenting the hemagglutinin of the H5N6 AIV was developed based on the ferritin antigen display platform. The results showed that a single-dose vaccination of this nanoparticle vaccine elicited potent hemagglutination inhibition (HI) antibody responses and neutralizing antibody responses in the chickens. Meanwhile, the fused HA-ferritin nanoparticle vaccine induced significantly higher levels of Th1/Th2 immune responses. After a lethal attack with the H5N6 virus, the fused HA-ferritin nanoparticle vaccine conferred chickens with 100 % (10/10) challenge protection. Importantly, the fused HA-ferritin nanoparticle with only 2 hemagglutination units (HAU) provided chickens with immune protection comparable to commercial inactivated vaccines and protected the chickens from severe lung pathological damage. These results in our study support the superiority of ferritin as an antigen display platform and suggest that self-assembled nanoparticle vaccines based on this platform possess the potential as an avian influenza candidate vaccine.
H5N6亚型高致病性禽流感病毒（HPAIV）的持续传播和演变对全球家禽业的发展以及人类公共卫生安全构成了挑战。为应对H5N6病毒的潜在威胁，一种安全有效的疫苗迫在眉睫。在我们的研究中，基于铁蛋白抗原展示平台开发了一种呈现H5N6禽流感病毒血凝素的自组装纳米颗粒疫苗。结果表明，单剂量接种这种纳米颗粒疫苗可在鸡体内引发强效血凝抑制（HI）抗体反应和中和抗体反应。同时，融合HA-铁蛋白纳米颗粒疫苗诱导产生了显著更高水平的Th1/Th2免疫反应。在用H5N6病毒进行致死性攻击后，融合HA-铁蛋白纳米颗粒疫苗为鸡提供了100%（10/10）的攻毒保护。重要的是，仅含2个血凝素单位（HAU）的融合HA-铁蛋白纳米颗粒为鸡提供了与商业灭活疫苗相当的免疫保护，并保护鸡免受严重的肺部病理损伤。我们研究中的这些结果支持了铁蛋白作为抗原展示平台的优越性，并表明基于该平台的自组装纳米颗粒疫苗具有作为禽流感候选疫苗的潜力。
