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水果保鲜中的先进技术:纳米级带电水颗粒对蓝莓贮藏品质和活性氧的影响

Advanced technology in fruit preservation: Effects of nanoscale charged water particles on storage quality and reactive oxygen species in blueberries.

作者信息

Yang Jiannan, Yin Jianting, Wang Kai, Zhao Lei, Yang Zhibiao, Cai Yingting, Lou Jiefeng, Huang Chao, Shen Qi

机构信息

Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, PR China; College of Food Science, South China Agricultural University, Guangzhou 510642, PR China.

Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, PR China; College of Food Science, South China Agricultural University, Guangzhou 510642, PR China.

出版信息

Food Res Int. 2024 Dec;198:115331. doi: 10.1016/j.foodres.2024.115331. Epub 2024 Nov 13.

Abstract

During the postharvest period, blueberries with a short shelf life due to microbial activity and an overload of reactive oxygen species (ROS) were still a major unresolved problem. In this study, the effect of nanoscale charged water particles (NCWP) treatment on the postharvest characteristics and ROS metabolism in blueberries (Vaccinium ashei Reade) were investigated. The results showed that NCWP treatment significantly inhibited microbial growth, maintained high firmness and commercial acceptability, and extended the storage period of blueberries. The nutrient of blueberries was retained and elevated after NCWP treatment, especially in the 6 d of NCWP-9 h treatment, the total phenol and anthocyanin content reached the peak at 565.1 mg/L and 5.26 mg/g, which contribute to the total antioxidant capacity of blueberries increased. SEM showed that NCWP-9 h treatment maintained the integrity of the cuticular wax of the blueberry peel, which indirectly decelerated the decline of blueberry firmness. The NCWP treatment significantly enhanced the antioxidant enzyme system of blueberry peel. On days 2, 4 and 6 after NCWP-9 h treatment, the CAT, SOD and APX activities were significantly different from the control group (P < 0. 05), with 585.09 ΔA/min/g, 79.34 U/g and 3.32 umol/min/g, respectively, which effectively scavenged the oxidative stress markers (HO, O) accumulated in the blueberry peels, and slowed down the aging and deteriorated of the blueberry process. This finding demonstrates that NCWP is an effective postharvest preservation method for blueberries and provides a viable strategy for quality maintenance in the postharvest fruit and vegetable sector.

摘要

在采后阶段,由于微生物活动和活性氧(ROS)过载导致蓝莓货架期较短,这仍是一个主要的未解决问题。本研究调查了纳米级带电水颗粒(NCWP)处理对蓝莓(Vaccinium ashei Reade)采后特性和ROS代谢的影响。结果表明,NCWP处理显著抑制微生物生长,保持高硬度和商业可接受性,并延长了蓝莓的贮藏期。NCWP处理后蓝莓的营养成分得以保留并提高,特别是在NCWP - 9小时处理6天时,总酚和花青素含量分别达到峰值565.1 mg/L和5.26 mg/g,这有助于蓝莓总抗氧化能力的提高。扫描电子显微镜(SEM)显示,NCWP - 9小时处理保持了蓝莓果皮角质蜡的完整性,这间接减缓了蓝莓硬度的下降。NCWP处理显著增强了蓝莓果皮的抗氧化酶系统。在NCWP - 9小时处理后的第2、4和6天,CAT、SOD和APX活性与对照组相比有显著差异(P < 0.05),分别为585.09 ΔA/min/g、79.34 U/g和3.32 umol/min/g,有效清除了蓝莓果皮中积累的氧化应激标记物(HO、O),并减缓了蓝莓衰老和变质的过程。这一发现表明,NCWP是一种有效的蓝莓采后保鲜方法,并为采后果蔬部门的品质维持提供了可行策略。

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