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用于研究小鼠大脑光遗传学刺激时正性或负性效价的光刺激回避高架十字迷宫实验方案。

Opto-Avoidance-Elevated Plus Maze protocol to study positive or negative valence upon optogenetic stimulation in the mouse brain.

作者信息

Serra Gian Pietro, Georges François, Wallén-Mackenzie Åsa

机构信息

Uppsala University, Department of Organism Biology, 752 36 Uppsala, Sweden; Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD 20815, USA.

Université de Bordeaux, Institut des Maladies Neurodégénératives, UMR 5293, 33000 Bordeaux, France; CNRS, Institut des Maladies Neurodégénératives, UMR 5293, 33000 Bordeaux, France.

出版信息

STAR Protoc. 2024 Dec 20;5(4):103474. doi: 10.1016/j.xpro.2024.103474. Epub 2024 Dec 6.

DOI:10.1016/j.xpro.2024.103474
PMID:39644496
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11667689/
Abstract

The elevated plus maze (EPM) apparatus consists of two open arms that provide aversive spaces and two closed arms that provide protective and welcoming spaces. Here, we present a protocol to implement the classical EPM apparatus in a real-time optogenetic environment to address behavioral avoidance in mice. We describe steps for performing stereotaxic surgery, mouse manipulation, and experimental setup. Finally, we detail the procedures required for the Opto-Avoidance-EPM test in which the naturally preferred closed arms are selectively paired with optogenetic stimulation. For complete details on the use and execution of this protocol, please refer to Serra et al..

摘要

高架十字迷宫（EPM）装置由两个提供厌恶空间的开放臂和两个提供保护性且宜人空间的封闭臂组成。在此，我们展示一种在实时光遗传学环境中实施经典EPM装置以解决小鼠行为回避问题的方案。我们描述了进行立体定位手术、小鼠操作和实验设置的步骤。最后，我们详细说明了光遗传学回避-EPM测试所需的程序，其中自然偏好的封闭臂与光遗传学刺激选择性配对。有关此方案使用和执行的完整详细信息，请参考塞拉等人的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3e6/11667689/f0c3bacb0e5b/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3e6/11667689/cd003b951c01/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3e6/11667689/8af5c104d60c/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3e6/11667689/69dcf013fa7f/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3e6/11667689/de5a5487c40b/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3e6/11667689/b8cbee91165b/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3e6/11667689/d57b3365b599/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3e6/11667689/f0c3bacb0e5b/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3e6/11667689/cd003b951c01/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3e6/11667689/8af5c104d60c/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3e6/11667689/69dcf013fa7f/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3e6/11667689/de5a5487c40b/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3e6/11667689/b8cbee91165b/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3e6/11667689/d57b3365b599/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3e6/11667689/f0c3bacb0e5b/gr6.jpg

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本文引用的文献

1
A role for the subthalamic nucleus in aversive learning.苍白球在厌恶学习中的作用。
Cell Rep. 2023 Nov 28;42(11):113328. doi: 10.1016/j.celrep.2023.113328. Epub 2023 Nov 4.
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Two Different Real-Time Place Preference Paradigms Using Optogenetics within the Ventral Tegmental Area of the Mouse.在小鼠腹侧被盖区使用光遗传学的两种不同实时位置偏好范式。
J Vis Exp. 2020 Feb 12(156). doi: 10.3791/60867.
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Optogenetics: 10 years of microbial opsins in neuroscience.光遗传学：微生物视蛋白在神经科学领域的十年
Nat Neurosci. 2015 Sep;18(9):1213-25. doi: 10.1038/nn.4091.
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Optogenetics.光遗传学。
Nat Methods. 2011 Jan;8(1):26-9. doi: 10.1038/nmeth.f.324. Epub 2010 Dec 20.
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A review of the validity and variability of the elevated plus-maze as an animal model of anxiety.高架十字迷宫作为焦虑动物模型的有效性和变异性综述。
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Validation of open:closed arm entries in an elevated plus-maze as a measure of anxiety in the rat.高架十字迷宫中开放臂与封闭臂进入次数作为大鼠焦虑指标的验证。
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