Shafferman A, Flashner Y, Cohen S
Mol Gen Genet. 1979 Oct 2;176(1):139-46. doi: 10.1007/BF00334305.
Protein synthesis and survival of colicinogenic bacteria carrying different ColE1 deletion and insertion mutants, were followed in presence or absence of the inducing agent mitomycin-C. It is concluded that active colicin is not involved in the process leading to death of the induced colicinogenic cell. The region of ColE1, essential for cell induced lethality, is carried on the DNA between map positions 0.74 to 0.27. In this region the DNA sequences carried between 0.74 to 0.79 and 0.0 to 0.27 are essential, while those located between 0.79 to 0.0 are nonessential for commitment to death. A cis interaction of the ColE1 DNA sequences in the essential regions is probably necessary for cell induced lethality. Some possibilities for such a cis interaction are suggested and discussed.
在有或没有诱导剂丝裂霉素-C的情况下，对携带不同ColE1缺失和插入突变体的产大肠杆菌素细菌的蛋白质合成和存活情况进行了跟踪研究。得出的结论是，活性大肠杆菌素不参与导致诱导产大肠杆菌素细胞死亡的过程。ColE1中对细胞诱导致死至关重要的区域位于图谱位置0.74至0.27之间的DNA上。在该区域中，位于0.74至0.79和0.0至0.27之间的DNA序列是必不可少的，而位于0.79至0.0之间的序列对于细胞死亡的发生并非必需。ColE1 DNA序列在必需区域的顺式相互作用可能是细胞诱导致死所必需的。文中提出并讨论了这种顺式相互作用的一些可能性。
