Liu Xiumei, Wang Weijun, Zhao Haitao, Wang Yongjie, Jiang Liming, Zhang Enshuo, Feng Yanwei, Wang Xumin, Qu Jiangyong, Yang Jianmin, Li Zan
College of Life Sciences, Yantai University, Yantai 264005, China.
Shandong Engineering Research Center of Oyster Germplasm Creation and Efficient Culture, Yantai 264025, China; School of Fisheries, Ludong University, Yantai 264025, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2025 Jun;54:101392. doi: 10.1016/j.cbd.2024.101392. Epub 2024 Dec 5.
As an important member of global aquaculture, oysters (Crassostrea gigas) have significant economic value. With the development of commercial aquaculture, the frequent occurrence of diseases caused by Vibrio alginolyticus has become a hindrance to high-density aquaculture. Gill tissue, as an important component of immune system of the oysters, plays the key point in the face of invasion by foreign substances. Compared to the diploid oyster, the triploid oyster presents a higher growth rate and lower growth investment, making it a more ideal model for studying oyster immune defense. In this study, triploid oysters were as the research subject, and gill tissues attacked by V. alginolyticus were sequenced. By analyzing samples from different time points, 1746 DEGs were obtained. The KEGG and GO functional enrichment analysis showed that gill tissues mainly participate in immune function through the PIK3-Akt signaling pathway and the MAPK signaling pathway. The protein interaction network revealed three genes (CASP8, CASP9 and PIK3CA) that play core roles in immune defense by analyzing the interaction relationship between genes. Finally, qRT-PCR verified the expression of key genes. This study provides a more effective scientific basis for disease prevention and control of oysters and other bivalve shellfish, and helps to promote the sustainable development of aquaculture.
作为全球水产养殖的重要成员，牡蛎（太平洋牡蛎）具有重要的经济价值。随着商业水产养殖的发展，由溶藻弧菌引起的疾病频繁发生已成为高密度养殖的障碍。鳃组织作为牡蛎免疫系统的重要组成部分，在面对外来物质入侵时起着关键作用。与二倍体牡蛎相比，三倍体牡蛎生长速度更快，生长投入更低，使其成为研究牡蛎免疫防御的更理想模型。本研究以三倍体牡蛎为研究对象，对受溶藻弧菌攻击的鳃组织进行测序。通过分析不同时间点的样本，获得了1746个差异表达基因（DEGs）。KEGG和GO功能富集分析表明，鳃组织主要通过PIK3-Akt信号通路和MAPK信号通路参与免疫功能。蛋白质相互作用网络通过分析基因之间的相互作用关系，揭示了三个在免疫防御中起核心作用的基因（CASP8、CASP9和PIK3CA）。最后，qRT-PCR验证了关键基因的表达。本研究为牡蛎及其他双壳贝类的疾病防控提供了更有效的科学依据，有助于促进水产养殖业的可持续发展。
