Johnson V E, Hilton P J
Clin Sci (Lond). 1985 Jan;68(1):89-91. doi: 10.1042/cs0680089.
Frusemide-sensitive sodium and potassium transport by normal human leucocytes has been studied in vitro by both isotopic and net flux techniques. In physiological media the leucocyte exhibits a frusemide-sensitive influx of sodium and potassium of equal magnitude compatible with a 1:1 co-transport system. Cells exposed to zero external sodium and potassium (osmolality maintained with choline) demonstrated a frusemide-sensitive sodium and potassium efflux. Frusemide-sensitive potassium influx was dependent on the presence of external sodium but frusemide-sensitive sodium influx persisted unchanged in the absence of external potassium. Frusemide-sensitive potassium influx was dependent on external chloride but frusemide-sensitive sodium influx was chloride-independent. These last two observations make it likely that the frusemide-sensitive pathway is capable of operating in modes other than sodium-potassium co-transport.
通过同位素和净通量技术，已在体外研究了正常人白细胞对速尿敏感的钠和钾转运。在生理介质中，白细胞表现出对速尿敏感的钠和钾内流，其大小相等，符合1:1协同转运系统。暴露于零外部钠和钾（用胆碱维持渗透压）的细胞表现出对速尿敏感的钠和钾外流。对速尿敏感的钾内流依赖于外部钠的存在，但在没有外部钾的情况下，对速尿敏感的钠内流保持不变。对速尿敏感的钾内流依赖于外部氯离子，但对速尿敏感的钠内流不依赖于氯离子。最后这两个观察结果表明，对速尿敏感的途径可能能够以钠钾协同转运以外的方式运作。
