A press-actuated slidable microfluidic colorimetric biosensor for Salmonella detection utilizing nickel mesh sheet and MIL-88@Pd/Pt nanoparticles.

A press-actuated slidable microfluidic colorimetric biosensor was designed for rapid, sensitive and multi-channel detection of Salmonella. The nickel mesh sheet (NMS) modified with capture antibodies was employed for capturing target bacteria, and metal organic frameworks decorated with palladium (Pd) and platinum (Pt) nanoparticles (MIL-88@Pd/Pt NPs) modified with detection antibodies were used for amplifying colorimetric signals. The capture efficiency of the immune NMS reached 83 %, and the detection limit of this colorimetric biosensor was 35 CFU/mL in 20 min. The average recoveries for Salmonella in spiked chicken meats ranged from 92.2 % to 102.5 % with a variation from 3.7 % to 7.2 %. The press-actuated slidable microfluidic chip was elaboratively developed with multiple functions, including mixing, separation, washing, catalysis and detection.