Yuan Jing, Wang Lei, Duan Hong, Ye Siyi, Ding Ying, Li Yanbin, Lin Jianhan
Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100083, China.
Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology & Business University, Beijing 100048, China.
Food Chem. 2025 Mar 1;467:142343. doi: 10.1016/j.foodchem.2024.142343. Epub 2024 Dec 4.
A press-actuated slidable microfluidic colorimetric biosensor was designed for rapid, sensitive and multi-channel detection of Salmonella. The nickel mesh sheet (NMS) modified with capture antibodies was employed for capturing target bacteria, and metal organic frameworks decorated with palladium (Pd) and platinum (Pt) nanoparticles (MIL-88@Pd/Pt NPs) modified with detection antibodies were used for amplifying colorimetric signals. The capture efficiency of the immune NMS reached 83 %, and the detection limit of this colorimetric biosensor was 35 CFU/mL in 20 min. The average recoveries for Salmonella in spiked chicken meats ranged from 92.2 % to 102.5 % with a variation from 3.7 % to 7.2 %. The press-actuated slidable microfluidic chip was elaboratively developed with multiple functions, including mixing, separation, washing, catalysis and detection.
设计了一种按压驱动的可滑动微流控比色生物传感器，用于快速、灵敏且多通道检测沙门氏菌。用捕获抗体修饰的镍网片（NMS）用于捕获目标细菌，用检测抗体修饰的、装饰有钯（Pd）和铂（Pt）纳米颗粒的金属有机框架（MIL-88@Pd/Pt NPs）用于放大比色信号。免疫NMS的捕获效率达到83%，该比色生物传感器在20分钟内的检测限为35 CFU/mL。加标鸡肉中沙门氏菌的平均回收率在92.2%至102.5%之间，变异系数在3.7%至7.2%之间。精心开发了具有混合、分离、洗涤、催化和检测等多种功能的按压驱动可滑动微流控芯片。
