一种手指驱动的微流控生物传感器，用于比色法检测食源性病原体。
A finger-actuated microfluidic biosensor for colorimetric detection of foodborne pathogens.
机构信息
Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100083, China.
Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA.
出版信息
Food Chem. 2022 Jul 1;381:131801. doi: 10.1016/j.foodchem.2021.131801. Epub 2021 Dec 8.
A microfluidic biosensor was developed for rapid detection of Salmonella using a finger-actuated micropump, a finger-actuated micromixer, gold@platinum nanocatalysts (Au@PtNCs) and a smartphone App. First, immune magnetic nanobeads (MNBs), bacterial sample and immune Au@PtNCs were successively finger-actuated pumped into microfluidic chip. Then, they were fully mixed using finger-actuated micromixer to form MNB-Salmonella-Au@PtNC complexes. After hydrogen peroxide-tetramethylbenzidine was pumped into chip and catalyzed by nanocatalysts on complexes, resulting in color change from colorless to blue, the image of catalysate was collected and finally analyzed by self-developed smartphone App or directly compared with Pantone color card in chip to determinebacterial concentration. Experimental results showed this biosensor could quantitatively detect Salmonella from 3.5 × 10 to 3.5 × 10 CFU/mL in 1 h with lower detection limit of 350 CFU/mL. This biosensor has successfully integrated loading, mixing, incubation, washing, separation and detection onto a chip and might pave a promising way for bacterial detection.
一种基于手指驱动微泵、手指驱动微混合器、金@铂纳米催化剂（Au@PtNCs）和智能手机应用程序的微流控生物传感器，用于快速检测沙门氏菌。首先，免疫磁性纳米珠（MNBs）、细菌样品和免疫 Au@PtNCs 被连续地用手指驱动微泵注入微流控芯片中。然后，使用手指驱动微混合器充分混合，形成 MNB-沙门氏菌-Au@PtNC 复合物。当过氧化氢-四甲基联苯胺被泵入芯片并被复合物上的纳米催化剂催化时，会发生从无色到蓝色的颜色变化，收集催化产物的图像，并最终通过自行开发的智能手机应用程序进行分析，或直接与芯片中的潘通色卡进行比较，以确定细菌浓度。实验结果表明，该生物传感器可以在 1 小时内定量检测 3.5×10 到 3.5×10 CFU/mL 的沙门氏菌，检测限低至 350 CFU/mL。该生物传感器已成功将加载、混合、孵育、洗涤、分离和检测集成到一个芯片上，为细菌检测开辟了一条有前途的道路。
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