Yoo Su Jin, Kim Hyeon Jin, Moon Hye Yun, Jeon Min-Seung, Cho Yong Uk, Jeon Che Ok, Eyun Seong-Il, Kang Hyun Ah
Department of Life Science, Chung-Ang University, Seoul 06974, South Korea.
J Agric Food Chem. 2024 Dec 18;72(50):28194-28208. doi: 10.1021/acs.jafc.4c08103. Epub 2024 Dec 9.
The importance of nonconventional yeasts has increasingly been highlighted, particularly for aroma formation in fermented foods. Here, we performed whole-genome sequencing of , which produces a variety of volatile flavor compounds, leading to the identification of the alcohol acyltransferase (AATase) family of genes. The genome of contains seven AATase genes, encoding alcohol--acetyltransferases (ATFs) and ethanol acetyltransferase 1 (EAT1) for acetate ester formation, along with ethanol hexanoyl transferase 1 (EHT1) for ethyl ester formation. Among five ATF homologues, only WsATF5 showed acetyltransferase activity toward myriocin, a structural analogue of sphingosine. In contrast, heterologous expression of WsEHT1 and WsEAT1 in promoted the production of ethyl decanoate and ethyl acetate, respectively, supporting their AATase activity. The enzymatic activity analyses revealed the additional alcoholysis activity of WsEAT1 and the thioesterase activity of WsEHT1. Subcellular localization analysis indicated that WsEAT1 was localized in the mitochondria, WsEHT1 in the endoplasmic reticulum and lipid droplets (LDs), and WsATF5 in the LDs. The novel AATases could be usefully applied to produce flavor components in various food industries.
非传统酵母的重要性日益凸显，尤其是在发酵食品的香气形成方面。在此，我们对能产生多种挥发性风味化合物的[具体名称未给出]进行了全基因组测序，从而鉴定出了醇酰基转移酶（AATase）基因家族。[具体名称未给出]的基因组包含七个AATase基因，其中编码用于乙酸酯形成的醇 - 乙酰转移酶（ATF）和乙醇乙酰转移酶1（EAT1），以及用于乙酯形成的乙醇己酰转移酶1（EHT1）。在五个ATF同源物中，只有WsATF5对鞘氨醇的结构类似物嗜热栖热放线菌素表现出乙酰转移酶活性。相比之下，WsEHT1和WsEAT1在[具体表达宿主未给出]中的异源表达分别促进了癸酸乙酯和乙酸乙酯的产生，证实了它们的AATase活性。酶活性分析揭示了WsEAT1的额外醇解活性和WsEHT1的硫酯酶活性。亚细胞定位分析表明，WsEAT1定位于线粒体，WsEHT1定位于内质网和脂滴（LDs），而WsATF5定位于脂滴。这些新型AATases可有效地应用于各种食品工业中风味成分的生产。
