Mattila H, Havurinne V, Antal T, Tyystjärvi E
Molecular Plant Biology, Department of Life Technologies, University of Turku, 20014 Turku, Finland.
Present address: ECOMARE, CESAM, Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal.
Photosynthetica. 2022 Nov 24;60(4):529-538. doi: 10.32615/ps.2022.049. eCollection 2022.
We recently developed a chlorophyll fluorescence method (activated F rise) for estimating if a light wavelength preferably excites PSI or PSII in plants. Here, the method was tested in green microalgae: , , , , , and . The species displayed a plant-like action spectra of F rise, suggesting that PSII/PSI absorption ratio is conserved from higher plants to green algae. F rise was weak in a strain of , , and . Interestingly, another strain exhibited a strong F rise. The result indicates that the same illumination can lead to different redox states of the plastoquinone pool in different algae. Flavodiiron activity enhanced the F rise, presumably by oxidizing the plastoquinone pool during pre-illumination. The activity of plastid terminal oxidase, in turn, diminished the F rise, but to a small degree.
我们最近开发了一种叶绿素荧光方法（激活的F上升），用于估计光波长是否优先激发植物中的光系统I（PSI）或光系统II（PSII）。在此，该方法在绿色微藻中进行了测试：、、、、、和。这些物种显示出类似植物的F上升作用光谱，表明从高等植物到绿藻，PSII/PSI吸收比是保守的。在、和的一个菌株中，F上升较弱。有趣的是，另一个菌株表现出强烈的F上升。结果表明，相同的光照可导致不同藻类中质体醌库的不同氧化还原状态。黄素二铁活性增强了F上升，可能是通过在预照明期间氧化质体醌库。相反，质体末端氧化酶的活性降低了F上升，但程度较小。
