Lee Rachel L, Meade Kieran G, Rhodes Shelley G, Ford Tom, Kyriazakis Ilias
Institute for Global Food Security, School of Biological Sciences, Queen's University, Belfast, United Kingdom BT9 5DL.
School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland D04 C1P1.
JDS Commun. 2024 May 10;5(6):622-627. doi: 10.3168/jdsc.2024-0547. eCollection 2024 Nov.
Investigations into the role of vitamin D (vitD) in the immune response of cattle are limited. The objectives of this study were therefore to investigate the association between circulating vitD concentration, tuberculosis (TB) vaccination, and infection outcomes in 24 dairy calves (<8 wk old) that were housed throughout and fed a BW-based allowance. The study design incorporated 2 phases: vaccination (experimental wk 0-52) and experimental infection phase (wk 52-65). Vaccinated calves (n = 12) received a subcutaneous injection of a live attenuated TB strain at wk 0, whereas unvaccinated ones (n = 12) were injected with saline. All animals were infected with 7,600 cfu of 52 wk postvaccination, and lung and lymph node tissues were assessed for pathology following euthanasia after wk 65. Blood samples were taken throughout wk 0 to 65. Serum 25-hydroxyvitamin D [25(OH)D] concentrations were measured using a commercial ELISA. A mixed-effects linear regression model revealed significantly higher 25(OH)D concentrations in the vaccinates postinfection (wk 65) compared with the nonvaccinates. Linear regression analysis between 25(OH)D concentration and the level of -driven pathology revealed a negative linear relationship (i.e., higher concentrations were associated with lower pathology scores) irrespective of vaccination status. No correlation was detected between IFN-γ cytokine production and vitD concentration. Overall, the results support an impactful role for vitD in the development of effective immunity of cattle against . Gaining insight into the interaction between TB vaccination, infection, and vitD could potentially guide the optimization of vaccination protocols and future TB control strategies.
关于维生素D（vitD）在牛免疫反应中作用的研究有限。因此，本研究的目的是调查24头全程饲养并按体重给予定量饲料的小于8周龄奶牛犊牛的循环vitD浓度、结核病（TB）疫苗接种与感染结果之间的关联。研究设计包括两个阶段：疫苗接种（实验第0 - 52周）和实验感染阶段（第52 - 65周）。接种疫苗的犊牛（n = 12）在第0周皮下注射减毒活结核菌株，而未接种疫苗的犊牛（n = 12）注射生理盐水。所有动物在接种疫苗52周后感染7600 cfu，在第65周安乐死后评估肺和淋巴结组织的病理学情况。在第0至65周期间采集血样。使用商业ELISA测定血清25 - 羟基维生素D [25(OH)D]浓度。混合效应线性回归模型显示，与未接种疫苗的犊牛相比，接种疫苗的犊牛在感染后（第65周）的25(OH)D浓度显著更高。25(OH)D浓度与γ干扰素驱动的病理学水平之间的线性回归分析显示存在负线性关系（即浓度越高，病理学评分越低），与疫苗接种状态无关。未检测到干扰素 - γ细胞因子产生与vitD浓度之间的相关性。总体而言，结果支持vitD在牛对结核病产生有效免疫的过程中发挥重要作用。深入了解结核病疫苗接种、感染与vitD之间的相互作用可能会为优化疫苗接种方案和未来的结核病控制策略提供指导。
