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从脆性X综合征患者的尿液样本中高效生成人类诱导多能干细胞。

Efficient generation of human induced pluripotent stem cells from urine samples of patients with Fragile X syndrome.

作者信息

Dionne Olivier, Sabatié Salomé, Fortin Fléchère, Corbin François, Laurent Benoit

机构信息

Department of Biochemistry and Functional Genomics, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, Canada.

Medical Genetics division, Centre Hospitalier Universitaire de Sherbrooke (CHUS), Sherbrooke, QC, Canada.

出版信息

Front Cell Dev Biol. 2024 Nov 22;12:1489190. doi: 10.3389/fcell.2024.1489190. eCollection 2024.

DOI:10.3389/fcell.2024.1489190
PMID:39650724
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11621072/
Abstract

Human induced pluripotent stem cells (iPSCs) are a valuable tool for studying human development and diseases. iPSCs can be generated by reprogramming from any somatic cells, however establishing primary cell cultures can involve invasive procedures (e.g., skin biopsy) and be labor-intensive. In this paper, we describe an efficient, reliable, and non-invasive method for cultivating primary urine-derived cells (UDCs) and efficiently reprogram them into iPSCs using a feeder-free and non-integrative system. This approach has several advantages: (i) UDCs collection and culture are non-invasive, straightforward, and do not require medical personnel; (ii) reprogramming UDCs using commercially available Sendai viruses is highly efficient and reliable; and (iii) iPSCs generated from UDCs demonstrate strong differentiation potential. To showcase the effectiveness of this method, we generated iPSC lines from UDCs of three control individuals and three patients with Fragile X syndrome.

摘要

人诱导多能干细胞（iPSC）是研究人类发育和疾病的宝贵工具。iPSC可以通过对任何体细胞进行重编程来产生，然而建立原代细胞培养可能涉及侵入性程序（例如皮肤活检）且劳动强度大。在本文中，我们描述了一种高效、可靠且非侵入性的方法，用于培养原代尿液衍生细胞（UDC），并使用无饲养层和非整合系统将其高效重编程为iPSC。这种方法有几个优点：（i）UDC的收集和培养是非侵入性的、直接的，并且不需要医务人员；（ii）使用市售仙台病毒对UDC进行重编程是高效且可靠的；（iii）由UDC产生的iPSC表现出强大的分化潜力。为了展示这种方法的有效性，我们从三名对照个体和三名脆性X综合征患者的UDC中生成了iPSC系。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/622a/11621072/b1d9ebb762bd/fcell-12-1489190-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/622a/11621072/0755ee4fb06d/fcell-12-1489190-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/622a/11621072/c0277d9b45eb/fcell-12-1489190-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/622a/11621072/30d80437043e/fcell-12-1489190-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/622a/11621072/d2e3d446b43e/fcell-12-1489190-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/622a/11621072/b3378e4e7d6d/fcell-12-1489190-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/622a/11621072/1e6e5cba95eb/fcell-12-1489190-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/622a/11621072/b1d9ebb762bd/fcell-12-1489190-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/622a/11621072/0755ee4fb06d/fcell-12-1489190-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/622a/11621072/c0277d9b45eb/fcell-12-1489190-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/622a/11621072/30d80437043e/fcell-12-1489190-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/622a/11621072/d2e3d446b43e/fcell-12-1489190-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/622a/11621072/b3378e4e7d6d/fcell-12-1489190-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/622a/11621072/1e6e5cba95eb/fcell-12-1489190-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/622a/11621072/b1d9ebb762bd/fcell-12-1489190-g007.jpg

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本文引用的文献

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Exploiting urine-derived induced pluripotent stem cells for advancing precision medicine in cell therapy, disease modeling, and drug testing.利用尿液来源的诱导多能干细胞推进细胞治疗、疾病建模和药物测试中的精准医学。
J Biomed Sci. 2024 May 9;31(1):47. doi: 10.1186/s12929-024-01035-4.
2
Induced pluripotent stem cells (iPSCs): molecular mechanisms of induction and applications.诱导多能干细胞（iPSCs）：诱导的分子机制与应用。
Signal Transduct Target Ther. 2024 Apr 26;9(1):112. doi: 10.1038/s41392-024-01809-0.
3
A short history of pluripotent stem cells markers.
多能干细胞标志物的简史。
Stem Cell Reports. 2024 Jan 9;19(1):1-10. doi: 10.1016/j.stemcr.2023.11.012. Epub 2023 Dec 28.
4
ISSCR standards for the use of human stem cells in basic research.国际干细胞研究学会人类干细胞基础研究使用标准。
Stem Cell Reports. 2023 Sep 12;18(9):1744-1752. doi: 10.1016/j.stemcr.2023.08.003.
5
Xeno-free culture and proliferation of hPSCs on 2D biomaterials.无动物培养体系和二维生物材料上 hPSC 的增殖。
Prog Mol Biol Transl Sci. 2023;199:63-107. doi: 10.1016/bs.pmbts.2023.02.008. Epub 2023 Mar 14.
6
Enhanced osteogenesis of human urine-derived stem cells by direct delivery of 30Kc19α-Lin28A protein.通过直接递送30Kc19α-Lin28A蛋白增强人尿液来源干细胞的成骨作用。
Front Bioeng Biotechnol. 2023 Jun 13;11:1215087. doi: 10.3389/fbioe.2023.1215087. eCollection 2023.
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Efficient generation of induced pluripotent stem cell lines from peripheral blood mononuclear cells.高效地从外周血单核细胞中生成诱导多能干细胞系。
Stem Cell Res. 2023 Jun;69:103088. doi: 10.1016/j.scr.2023.103088. Epub 2023 Mar 28.
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Advances in the application of induced pluripotent stem cells in pediatric diseases.诱导多能干细胞在儿科疾病中的应用进展。
Yi Chuan. 2023 Jan 20;45(1):42-51. doi: 10.16288/j.yczz.22-245.
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Characterization of human induced pluripotent stems cells: Current approaches, challenges, and future solutions.人类诱导多能干细胞的特征：当前方法、挑战及未来解决方案
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