一种通过液相色谱从尿毒症血浆和正常尿液中分离出来并经核磁共振鉴定的化合物。
A compound from uremic plasma and from normal urine isolated by liquid chromatography and identified by nuclear magnetic resonance.
作者信息
Gallice P, Monti J P, Crevat A, Durand C, Murisasco A
出版信息
Clin Chem. 1985 Jan;31(1):30-4.
A compound present in normal urine and in ultrafiltrates of uremic plasma in the fraction of so-called "uremic middle molecules" was isolated by liquid chromatography. Preliminary studies, including amino acid analysis, characterization of uronic acids, and ultraviolet spectroscopy, show that the molecule contains glycine, a uronic acid, and an aromatic ring. Characterization by 1H and 13C nuclear magnetic resonance spectrometry shows conclusively that this compound is a double conjugate of glucuronidate--o-hydroxyhippuric acid, which has been previously described by Zimmerman et al., using quite different techniques of isolation and identification (Clin Nephrol 14: 107, 1980; FEBS Lett 129: 237, 1981).
通过液相色谱法从正常尿液以及尿毒症血浆超滤液中的所谓“尿毒症中分子”部分分离出一种化合物。包括氨基酸分析、糖醛酸表征和紫外光谱分析在内的初步研究表明，该分子含有甘氨酸、一种糖醛酸和一个芳香环。通过氢核磁共振光谱和碳核磁共振光谱进行的表征最终表明，这种化合物是葡萄糖醛酸酯 - 邻羟基马尿酸的双共轭物，齐默尔曼等人此前曾使用完全不同的分离和鉴定技术描述过该化合物（《临床肾脏病学》14: 107, 1980；《欧洲生物化学学会联合会快报》129: 237, 1981）。
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