Leissing N, Izzo R, Sargent H
Clin Chem. 1985 Jan;31(1):83-6.
Variance components and individuality ratios for each of 25 chemical constituents of serum were estimated in a population of beagles. Ten blood samples were obtained from each of 14 beagles, seven of each sex, approximately weekly during three months. Glucose and phospholipid displayed marked inter-dog variation, as compared with intra-dog variation (individuality ratio less than 1). Triglycerides and nonesterified fatty acids had large intra-dog variances relative to their inter-dog variances (individuality ratio greater than 1). Overall, the estimated individuality ratios exceeded 1 for 20 of the 25 constituents. We conclude that subtraction of pretreatment values in the statistical analysis of the data from assays with individuality ratios greater than 1 may mask small clinically or biologically important changes between treatment groups. When the individuality ratio is greater than 1, pretreatment values should only be used as a population screening tool before a study.
在一组比格犬中估计了血清中25种化学成分各自的方差成分和个体比率。从14只比格犬（每种性别各7只）中大约每周采集一次血样，共采集三个月，每次从每只犬采集10份血样。与犬只内部变异相比，葡萄糖和磷脂显示出明显的犬只间变异（个体比率小于1）。甘油三酯和非酯化脂肪酸相对于其犬只间方差具有较大的犬只内方差（个体比率大于1）。总体而言，25种成分中有20种的估计个体比率超过1。我们得出结论，在对个体比率大于1的检测数据进行统计分析时，减去预处理值可能会掩盖治疗组之间临床上或生物学上的小的重要变化。当个体比率大于1时，预处理值仅应在研究前用作群体筛选工具。
