Pickup J F, Harris E K, Kearns M, Brown S S
Clin Chem. 1977 May;23(5):842-50.
The advent of high-capacity multi-channel analyzers allows estimation of long-term variability in serum constituents of large numbers of subjects. By frozen storage of specimens with subsequent analysis in a single machine run, long-term analytical variation may be eliminated, thus sharpening the estimates of intra-individual variation. In the present study we used the Vickers M-300 analyzer to obtain the data for such estimates from 37 male volunteers, each bled once a week for 22 weeks. Secimens were analyzed in random order to eliminate any biasing effect of analytical drift during the 4-h machine run. Ten serum constituents were measured. Storage-induced linear trends were small or negligible during the period of specimen collection. Using the ratio of average within-subject variance to the variance among subjects as a guide, serum alkaline phosphatase was found to show the greatest individuality, sodium and potassium the least. Other constitutents showed varying degrees of individuality, but for all these analytes, the usual population-based reference ranges were found to be either insensitive or irrelevant to the study of concentration changes over time within most healthy subjects. Our results generally confirmed those of smaller but comparable earlier studies.
高容量多通道分析仪的出现使得对大量受试者血清成分的长期变异性进行评估成为可能。通过冷冻保存标本并在单次机器运行中进行后续分析，可以消除长期分析变异，从而更精确地估计个体内变异。在本研究中，我们使用维氏M - 300分析仪从37名男性志愿者那里获取此类估计数据，每位志愿者每周采血一次，共采血22周。标本按随机顺序进行分析，以消除4小时机器运行期间分析漂移的任何偏倚效应。测量了十种血清成分。在标本采集期间，储存诱导的线性趋势很小或可忽略不计。以个体内平均方差与个体间方差之比为指导，发现血清碱性磷酸酶表现出最大的个体差异，而钠和钾的个体差异最小。其他成分表现出不同程度的个体差异，但对于所有这些分析物，通常基于总体的参考范围被发现对于大多数健康受试者随时间的浓度变化研究要么不敏感，要么不相关。我们的结果总体上证实了早期规模较小但类似研究的结果。
