Sheikh S P, Baldissera F G, Karlsen F O, Holst J J
FEBS Lett. 1985 Jan 1;179(1):1-6. doi: 10.1016/0014-5793(85)80178-2.
Specimens from porcine pancreas and ileal mucosa were extracted in acid/ethanol, subjected to gel permeation chromatography, ion-exchange chromatography, enzymatic peptide degradation, reverse-phase HPLC, and analysed for glucagon-like and glicentin-like immunoreactivity by region-specific radioimmunoassays. Results obtained with all methods were consistent with the hypothesis that glicentin is present in the pig pancreas in small amounts.
从猪胰腺和回肠黏膜中提取的样本用酸/乙醇进行萃取，然后进行凝胶渗透色谱法、离子交换色谱法、酶促肽降解、反相高效液相色谱法处理，并通过区域特异性放射免疫分析法分析胰高血糖素样和甘丙肽样免疫反应性。所有方法获得的结果均与猪胰腺中存在少量甘丙肽这一假设相符。
