Xu Cheng, Li Junbo, Liu Jian, Ma Yaxin, Wang Xiaoshuai, Xie Yunfei, Chen Chunxia, Ji Wei
Center for Innovative Research in Synthetic Chemistry and Resource Utilization, College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin, 150040, China.
Center for Innovative Research in Synthetic Chemistry and Resource Utilization, College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin, 150040, China.
Biosens Bioelectron. 2025 Mar 1;271:117044. doi: 10.1016/j.bios.2024.117044. Epub 2024 Dec 5.
A rapid, low-cost and reliable interfacial plasmonic nanoarray is presented as surface-enhanced Raman scattering (SERS) sensing platform for preliminary quantification and identification of flavonoids. Here, CTAB-modified Au colloidal nanoparticles self-assemble at the cyclohexane/acetone-water interface to form a uniform interfacial plasmonic nanoarray. The target hydrophobic analytes including organic dye methyl red and water-insoluble flavonoids, are effectively captured at the air-water interface and enter the "hot spots" between nanoparticles during the evaporation of the oil phase, which contributes to sensitive and reproducible SERS signals. Furthermore, this remarkable SERS performance enables the quantitative determination of water-insoluble flavonoids such as kaempferol, luteolin and naringenin with low detection limits of 10 M, and an approximately linear correlation between SERS signals and analytical concentrations, as well as rapid multiplex analysis of flavonoids with similar structural characteristics. Additionally, directly relative content detection of crude extracts from lingonberry (Vaccinium vitis-idaea L.) is achieved on the plasmonic nanoarray, serving as a proof-of-concept demonstration for practical applications. Compared to conventional analyses of flavonoids, the proposed SERS platform circumvents complex and time-consuming pretreatments, thereby opening avenues for the analysis of oil-soluble samples and other secondary metabolites, which will facilitate widespread evaluation of quality and medical value.
一种快速、低成本且可靠的界面等离子体纳米阵列被用作表面增强拉曼散射（SERS）传感平台，用于黄酮类化合物的初步定量和鉴定。在此，十六烷基三甲基溴化铵（CTAB）修饰的金胶体纳米颗粒在环己烷/丙酮 - 水界面自组装，形成均匀的界面等离子体纳米阵列。目标疏水性分析物，包括有机染料甲基红和水不溶性黄酮类化合物，在空气 - 水界面被有效捕获，并在油相蒸发过程中进入纳米颗粒之间的“热点”，这有助于产生灵敏且可重复的SERS信号。此外，这种卓越的SERS性能能够对水不溶性黄酮类化合物如槲皮素、木犀草素和柚皮苷进行定量测定，检测限低至10⁻⁶ M，SERS信号与分析浓度之间呈现近似线性关系，还能对具有相似结构特征的黄酮类化合物进行快速多重分析。此外，在等离子体纳米阵列上实现了对越橘（Vaccinium vitis - idaea L.）粗提物中直接相关含量检测，作为实际应用的概念验证示范。与传统的黄酮类化合物分析相比，所提出的SERS平台避免了复杂且耗时的预处理，从而为油溶性样品和其他次生代谢物的分析开辟了途径，这将有助于对质量和医学价值进行广泛评估。
