一种简单快速的DNA测序方法。
A simple and rapid method for sequencing DNA.
作者信息
Chuvpilo S A, Kravchenko V V
出版信息
FEBS Lett. 1985 Jan 1;179(1):34-6. doi: 10.1016/0014-5793(85)80185-x.
A simplified technique for DNA sequence analysis has been developed, based on modification of a previous method [(1980) Methods Enzymol. 65, 499-560]. It employs an adsorptive immobilization of terminally labelled DNA on DEAE paper followed by G, A+G, C+T and C specific modification and cleavage reactions. This solid-phase technique is faster and more convenient than the original method. The efficiency is comparable. The total processing time taken to produce cleaved fragments loaded on a gel is less than 2 h.
基于对先前方法的改进（[(1980) Methods Enzymol. 65, 499 - 560]），已开发出一种简化的DNA序列分析技术。该技术采用将末端标记的DNA吸附固定在DEAE纸上，随后进行G、A + G、C + T和C特异性修饰及切割反应。这种固相技术比原始方法更快、更方便。效率相当。产生加载到凝胶上的切割片段所需的总处理时间少于2小时。
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