一种用于确定胸腺嘧啶（T）残基的光诱导DNA切割。
A photoinduced cleavage of DNA useful for determining T residues.
作者信息
Simoncsits A, Török I
出版信息
Nucleic Acids Res. 1982 Dec 20;10(24):7959-64. doi: 10.1093/nar/10.24.7959.
Abstract
Irradiation of 5'-[32P]-phosphate labeled DNA fragments with ultraviolet light in the presence of primary amines followed by piperidine treatment resulted in base-specific cleavage of the DNA chain at T residues, accompanied by a less intensive G reaction. This simple, T greater than G cleavage offers an alternative method for determining T residues in chemical DNA sequencing.
摘要
在伯胺存在的情况下，用紫外线照射5'-[32P]-磷酸标记的DNA片段，随后进行哌啶处理，导致DNA链在T残基处发生碱基特异性切割，并伴有强度较低的G反应。这种简单的、T大于G的切割为化学DNA测序中确定T残基提供了一种替代方法。
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