Li Jiaxin, Hao Gaimei, Yan Yupeng, Li Ming, Li Gaifen, Lu Zhengmin, Sun Zhibo, Chen Yanjing, Liu Haixia, Zhao Yukun, Wu Meng, Bao Xiangxin, Wang Yong, Li Yubo
Institute of Basic Theory for Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
Xuanwu Hospital, Capital Medical University, Beijing, 100053, China.
Neurochem Int. 2025 Jan;182:105914. doi: 10.1016/j.neuint.2024.105914. Epub 2024 Dec 7.
The field of hydrogen medicine has garnered extensive attention since Professor Ohsawa established that low concentrations of hydrogen (2%-4%) exert antioxidant effects. The present study aimed to evaluate the therapeutic effect of molecular hydrogen in a CUMS rat model.
A total of 40 SD rats were randomly divided into a control group, a model group, a hydrogen group, and a positive drug group. Four weeks post-modeling, hydrogen inhalation and other treatments were administered. Behavioral, biochemical, and immunohistochemical evaluations were performed after treatment.
Hydrogen inhalation alleviated depressive behavior and hippocampal neuronal damage in CUMS rats, as well as restored the levels of neurotransmitters, inflammatory factors, and oxidative stress. Moreover, it maintained mitochondrial homeostasis and up-regulated the expression of PGC-1α, PINK1, and Parkin.
The results collectively indicated that hydrogen significantly attenuated CUMS-induced depressive-like behavior and monoamine neurotransmitter deficiency, as well as protected the brain from oxidative stress and inflammatory damage and effectively preserved mitochondrial homeostasis.
自从大泽教授证实低浓度氢气（2%-4%）具有抗氧化作用以来，氢医学领域受到了广泛关注。本研究旨在评估分子氢对慢性不可预测温和应激（CUMS）大鼠模型的治疗效果。
将40只SD大鼠随机分为对照组、模型组、氢气组和阳性药物组。建模四周后，进行氢气吸入及其他处理。处理后进行行为学、生物化学和免疫组织化学评估。
氢气吸入减轻了CUMS大鼠的抑郁行为和海马神经元损伤，恢复了神经递质、炎症因子和氧化应激水平。此外，它维持了线粒体稳态，并上调了PGC-1α、PINK1和Parkin的表达。
结果共同表明，氢气显著减轻了CUMS诱导的抑郁样行为和单胺神经递质缺乏，保护大脑免受氧化应激和炎症损伤，并有效维持线粒体稳态。
