Budzinski Lisa, Sempert Toni, Lietz Leonie, Maier René, Kang Gi-Ung, von Stuckrad Anne Sae Lim, Goetzke Carl Christoph, Roth Maria, Shah Aayushi, Abbas Amro, Lehman Katrin, Necke Kathleen, Bartsch Stefanie, Hoffmann Ute, Mashreghi Mir-Farzin, Biesen Robert, Kallinich Tilmann, Chang Hyun-Dong
German Rheumatology Research Center Berlin - A Leibniz Institute, Charitéplatz 1, Berlin, 10117, Germany.
Department for Cytometry, Institute of Biotechnology, Technische Universität Berlin, Berlin, Germany.
Mol Cell Pediatr. 2024 Dec 10;11(1):12. doi: 10.1186/s40348-024-00186-6.
Juvenile Idiopathic Arthritis (JIA) comprises diverse chronic inflammatory conditions driven by malfunction of the immune system. The intestinal microbiota is considered a crucial environmental factor correlating with chronic inflammatory diseases, and for JIA certain alterations in the microbiota have already been described.
Here, we have characterized intestinal microbiota samples from 54 JIA patients and 38 pediatric healthy controls by conventional 16S rRNA sequencing and by single-cell analysis for phenotypic features by multi-parameter microbiota flow cytometry (mMFC), which complements the population-based taxonomic profiling with the characterization of individual bacterial cells.
We found age to be a crucial confounder in microbiota analyses of JIA patients. Age stratification revealed specific microbiota alterations neglected by the general comparison of JIA patients and pediatric controls.
Age groups presented distinct taxonomic profiles and microbiota phenotypic signatures which transitioned with age, highlighting changes in the microbiota-immune system interaction with age.
青少年特发性关节炎（JIA）包括由免疫系统功能失调驱动的多种慢性炎症性疾病。肠道微生物群被认为是与慢性炎症性疾病相关的关键环境因素，并且已经描述了JIA患者微生物群的某些改变。
在这里，我们通过传统的16S rRNA测序以及通过多参数微生物群流式细胞术（mMFC）对表型特征进行单细胞分析，对54例JIA患者和38例儿科健康对照的肠道微生物群样本进行了表征，该方法通过个体细菌细胞的表征补充了基于群体的分类学分析。
我们发现年龄是JIA患者微生物群分析中的一个关键混杂因素。年龄分层揭示了JIA患者与儿科对照总体比较所忽略的特定微生物群改变。
年龄组呈现出不同的分类学特征和微生物群表型特征，这些特征随年龄而转变，突出了微生物群与免疫系统相互作用随年龄的变化。
