Koonce M P, Schliwa M
J Cell Biol. 1985 Jan;100(1):322-6. doi: 10.1083/jcb.100.1.322.
Intracellular organelle transport was studied in a new model system, the giant freshwater ameba Reticulomyxa. The ameba extends a large reticulate network of cytoplasmic strands in which various phase-dense organelles can be seen to move at a rate of up to 25 microns/s. This combined light and high voltage electron microscopic study shows that organelles move bidirectionally in even the finest network strands that contain only a single microtubule. In terms of microtubule-associated intracellular transport, this observation defines a minimum set of conditions necessary for such movement. The implications of this finding for possible models of force generation are discussed.
在一种新的模型系统——大型淡水变形虫网柄菌中研究了细胞内细胞器运输。这种变形虫伸出一个由细胞质丝组成的大型网状网络,在其中可以看到各种致密相细胞器以高达25微米/秒的速度移动。这项结合了光学显微镜和高压电子显微镜的研究表明,即使在仅包含单个微管的最细网络丝中,细胞器也能双向移动。就微管相关的细胞内运输而言,这一观察结果定义了这种移动所需的一组最低条件。讨论了这一发现对可能的力产生模型的影响。