一些与运动相关的微管的极性

Euteneuer U, McIntosh J R

Proc Natl Acad Sci U S A. 1981 Jan;78(1):372-6. doi: 10.1073/pnas.78.1.372.

We have investigated the structural polarity of microtubules from several systems in which these fibers are thought to contribute to cell motility. By using a method for displaying microtubule polarity in the electron microscope, we find that both the A and B subfibers of Tetrahymena ciliary outer doublets and the inner pair of single microtubules are all oriented with their plus ends (i.e., their fast-growing ends) distal to the basal body. All of the microtubules in the axopodia of the heliozoan Actinosphaerium and all of the microtubules in the processes of melanophores from the angelfish Pterophyllum are likewise oriented with their plus ends distal to the cell centers. These results suggest that cellular systems for motility, and even those capable of bidirectional motility, can be constructed from microtubules of a single polarity.

我们研究了来自多个系统的微管的结构极性，在这些系统中，这些纤维被认为有助于细胞运动。通过使用一种在电子显微镜下显示微管极性的方法，我们发现四膜虫纤毛外双联体的A和B亚纤维以及内部的单微管对，其正端（即它们快速生长的末端）都朝向基体的远端。太阳虫放射太阳虫轴足中的所有微管以及神仙鱼蝶翼鱼黑素细胞突起中的所有微管同样以其正端朝向细胞中心的远端。这些结果表明，细胞运动系统，甚至那些能够双向运动的系统，可以由单一极性的微管构建而成。