Ma Haigang, Yu Yinshi, Zhu Yahui, Wu Hongjun, Qiu Haixia, Gu Ying, Chen Qian, Zuo Chao
Smart Computational Imaging Laboratory (SCILab), School of Electronic and Optical Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu 210094, China.
Smart Computational Imaging Research Institute (SCIRI) of Nanjing University of Science and Technology, Nanjing, Jiangsu 210019, China.
Photoacoustics. 2024 Nov 7;41:100664. doi: 10.1016/j.pacs.2024.100664. eCollection 2025 Feb.
Monitoring of microvascular calcification (MC) is essential for the understanding of pathophysiological processes and the characterization of certain physiological states such as drug abuse, metabolic abnormality, and chronic nephrosis. In this work, we develop a novel and effective time-resolved photoacoustic microscopy (TR-PAM) technology, which can observe the obvious microvascular bio-elastic change in the development process of the MC owing to the calcium deposition along vascular walls.The feasibility of the TR-PAM imaging was validated using a group of agar phantoms and tissues. Furthermore, MC pathological animal models were constructed and imaged and by the TR-PAM to demonstrate its capability for the bio-mechanical monitoring and characterization of MC, and experimental results were consistent with the pathological knowledge. The feasibility study of monitoring MC by the TR-PAM proves that this technique has potential to be developed as a superficial microvascular bio-mechanical assessment method to supplement current clinical strategy for prediction and monitoring of some diseases.
监测微血管钙化（MC）对于理解病理生理过程以及某些生理状态（如药物滥用、代谢异常和慢性肾病）的特征描述至关重要。在这项工作中，我们开发了一种新颖且有效的时间分辨光声显微镜（TR-PAM）技术，由于沿血管壁的钙沉积，该技术可以观察到MC发展过程中明显的微血管生物弹性变化。使用一组琼脂仿体和组织验证了TR-PAM成像的可行性。此外，构建了MC病理动物模型并通过TR-PAM进行成像，以证明其对MC进行生物力学监测和特征描述的能力，实验结果与病理学知识一致。通过TR-PAM监测MC的可行性研究证明，该技术有潜力发展成为一种浅表微血管生物力学评估方法，以补充当前用于某些疾病预测和监测的临床策略。
